Microbiology Methods for ERP Review 3-2020

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AOAC Official Method 2020.xxx Salmonella species in a Broad Range of Foods and Select Environmental Samples: GENE-UP ® Salmonella (SLM) Test Method First Action 2020 Applicable to detection of Salmonella in 25 g test portions of fresh raw ground chicken, fresh raw chicken breast, fresh raw ground beef (80% lean), fresh raw fish, creamy peanut butter, vanilla ice cream, dry pet food and milk chocolate; for 30 mL test portions of chicken carcass rinse; for 100 g test portions of pasteurized liquid eggs and powdered egg; for 375 g test portions of fresh raw ground beef (80% lean), raw beef trim, raw ground pork, raw ground bison, whey protein powder, dry pet food, creamy peanut butter, garlic powder, bulk bagged Romaine lettuce, dark chocolate and milk chocolate; for stainless steel environmental sponges.

See Table 2020.1A-B for a summary of results of the inter-laboratory study. See Table 2020.2A-B for detailed results of the inter-laboratory study

A. Principle

The GENE-UP ® Salmonella (SLM) kit is designed for use with the GENE-UP ® Thermocycler. The GENE-UP ® SLM kit contains all of the necessary components for PCR, including sample-specific primers and probes and an internal amplification control. The GENE- UP ® Thermocycler detects fluorescence at several wavelengths (channels) to allow for multi- target detection in the same reaction vessel. The fluorescent signal from a sample is recorded in channel 640, while the fluorescent signal for an internal amplification control is recorded in channel 705. The software automatically interprets the results for the internal amplification

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