Microbiology Methods for ERP Review 3-2020

132

GENE-UP SLM Matrix Extension

June 19, 2017

reference methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods 1 (10 and 24 h). The >10 CFU level samples resulted in 5 positives out of 5 replicates for both methods (10 2 and 24 h). For the 0.29 CFU level of inoculation, the method had 5 out of 20 samples presumptive and 3 confirmed (by both alternative and reference methods) positives after both 10 and 24 hours. There were 5 4 out of 20 positives for the MLG reference method. There was no significant difference between the GENE ‐ 5 UP Salmonella detection assay presumptive and the confirmed or between the GENE ‐ UP method and the 6 reference method results using probability of detection (POD) statistical model (see Tables 7 ‐ 9). 7 8 Powdered egg : Powdered egg had <10 CFU/g of background organisms and was negative for natural 9 occurring Salmonella . Analysis was performed on three levels of S.enterica serovar Abony inoculation: 0 10 and 1.21 (0.69, 2.38) and >10 (4, >10) MPN/test portion for the GENE ‐ UP and the BAM reference methods. 11 The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. The >10 CFU level 12 samples resulted in 5 positives out of 5 replicates for both methods. For the 1.21 CFU level of inoculation, 13 the method had 17 out of 20 samples presumptive and confirmed (by both alternative and reference 14 methods) positives and 15 out of 20 for the BAM reference method. There was no significant difference 15 between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or between the GENE ‐ 16 UP method and the reference method results using probability of detection (POD) statistical model (see 17 Tables 7 ‐ 9). 18 19 Whey protein : Whey protein had 100 CFU/g of background organisms and was negative for natural 20 occurring Salmonella . Analysis was performed on three levels of S.enterica serovar Newport inoculation: 0 21 and 0.69 (0.53, 1.53) and >10 (2.34, >10) MPN/test portion for the GENE ‐ UP and the BAM reference 22 methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. The >10 23 CFU level samples resulted in 5 positives out of 5 replicates for both methods. For the 0.69 CFU level of 24 inoculation, the method had 9 out of 20 samples presumptive and confirmed (by both alternative and 25 reference methods) positives and 14 out of 20 for the BAM reference method. There was no significant 26 difference between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or between 27 the GENE ‐ UP method and the reference method results using probability of detection (POD) statistical 28 model (see Tables 10 ‐ 12). 29 30 Nonfat dried milk : Nonfat dried milk had >2500 CFU/g of background organisms and was negative for 31 natural occurring Salmonella . Analysis was performed on three levels of S.enterica serovar Salford 32 inoculation: 0 and 0.38 (0.16, 0.58) and 1.21 (0.68, >10) MPN/test portion for the GENE ‐ UP and the BAM 33 reference methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. 34 The 1.21 CFU level samples resulted in 5 positives out of 5 replicates for both methods. For the 0.38 CFU 35 level of inoculation, the method had 4 out of 20 samples presumptive and confirmed (by both alternative 36 and reference methods) positives and 5 out of 20 for the BAM reference method. There was no significant 37 difference between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or between 38 the GENE ‐ UP method and the reference method results using probability of detection (POD) statistical 39 model (see Tables 10 ‐ 12). 40 41 Dry pet food : Dry pet food had <10 CFU/g of background organisms and was negative for natural occurring 42 Salmonella . Analysis was performed on three levels of S.enterica serovar Paratyphi B inoculation: 0 and 43 0.17 (0.10, 0.40) and 0.69 (0.37, 4.38) MPN/test portion for the GENE ‐ UP and the BAM reference methods. 44 The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. The 0.69 CFU level 45 samples resulted in 4 positives out of 5 replicates for the GENE UP method and 5 out of 5 positives for the 46 BAM method. For the 0.17 CFU level of inoculation, the method had 4 out of 20 samples presumptive and 47 confirmed (by both alternative and reference methods) positives and 5 out of 20 for the BAM reference 48 method. There was no significant difference between the GENE ‐ UP Salmonella detection assay 49

Page 17 of 33