Microbiology Methods for ERP Review 3-2020

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GENE-UP SLM Matrix Extension

June 19, 2017

or between the GENE ‐ UP method and the reference method results using probability of detection (POD) 1 statistical model (see Tables 13 ‐ 15). 2 3 Beef trim: Beef trim had 4.3 x 10 6 CFU/g of background organisms and was negative for natural occurring 4 Salmonella . Analysis was performed on three levels of S.enterica serovar Hadar inoculation: 0, 0.39 (0.18, 5 0.68) and 3.49 (1.13, 10.74) MPN/test portion for the GENE ‐ UP and the MLG reference methods using both 6 BPW and mTSB primary enrichment media. The un ‐ inoculated samples resulted in 0 positives out of 5 7 replicates for both methods (BPW and mTSB, 10 and 24 h). The 3.49 CFU level samples resulted in 5 8 positives out of 5 replicates for both methods (BPW and mTSB, 10 and 24 h). For the 0.39 CFU level of 9 inoculation using BPW, the method had 7 out of 20 samples presumptive and confirmed (by both 10 alternative and reference methods) positives after both 10 and 24 h. For the 0.39 CFU level of inoculation 11 using mTSB, the method had 3 out of 20 samples presumptive positives after 10 h. There were 4 12 presumptive and confirmed positives. There were 5 out of 20 positives for the MLG reference method. 13 There was no significant difference between the GENE ‐ UP Salmonella detection assay presumptive and the 14 confirmed or between the GENE ‐ UP method and the reference method results using probability of 15 detection (POD) statistical model (see Tables 13 ‐ 15). 16 17 Pasteurized liquid egg: Pasteurized liquid egg had <10 CFU/g of background organisms and was negative for 18 natural occurring Salmonella . Analysis was performed on three levels of S.enterica serovar Typhimurium 19 inoculation: 0 and 0.36 (0.17, 0.64) and 2.19 (0.94, 5.13) MPN/test portion for the GENE ‐ UP and the MLG 20 reference methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. 21 The 2.19 CFU level samples resulted in 5 positives out of 5 replicates for both methods. For the 0.36 CFU 22 level of inoculation, the method had 6 out of 20 samples presumptive and confirmed (by both alternative 23 and reference methods) positives and 5 out of 20 for the MLG reference method. There was no significant 24 difference between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or between 25 the GENE ‐ UP method and the reference method results using probability of detection (POD) statistical 26 model (see Tables 13 ‐ 15). 27 28 Creamy peanut butter : Creamy peanut butter had 1.8 x 10 3 CFU/g of background organisms and was 29 negative for natural occurring Salmonella . Analysis was performed on three levels of S.enterica serovar 30 Potsdam inoculation: 0 and 0.69 (0.40, 1.14) and 5.42 (1.61, 18.25) MPN/test portion for the GENE ‐ UP and 31 the BAM reference methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both 32 methods. The 5.42 CFU level samples resulted in 5 positives out of 5 replicates for both methods. For the 33 0.69 CFU level of inoculation, the method had 11 out of 20 samples presumptive and confirmed (by both 34 alternative and reference methods) positives and 9 out of 20 for the BAM reference method. There was no 35 significant difference between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or 36 between the GENE ‐ UP method and the reference method results using probability of detection (POD) 37 statistical model (see Tables 13 ‐ 15). 38 39 Garlic powder : Garlic powder had 3.6 x 10 3 CFU/g of background organisms and was negative for natural 40 occurring Salmonella . Analysis was performed on three levels of S.enterica serovar Arizonae inoculation: 0 41 and 0.45 (0.22, 0.78) and 3.49 (1.13, 10.74) MPN/test portion for the GENE ‐ UP and the BAM reference 42 methods. The un ‐ inoculated samples resulted in 0 positives out of 5 replicates for both methods. The 3.49 43 CFU level samples resulted in 5 positives out of 5 replicates for both methods. For the 0.45 CFU level of 44 inoculation, the method had 6 out of 20 samples presumptive and confirmed (by both alternative and 45 reference methods) positives and 7 out of 20 for the BAM reference method. There was no significant 46 difference between the GENE ‐ UP Salmonella detection assay presumptive and the confirmed or between 47 the GENE ‐ UP method and the reference method results using probability of detection (POD) statistical 48 model (see Tables 13 ‐ 15). 49

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