Microbiology Methods for ERP Review 3-2020

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dPOD C e 95% CI f

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Un ‐ inoculated 5 0 0.00 0.00, 0.43 0 0 0.00, 0.43 0.00 ‐ 0.43, 0.43

Low 20 17 0.85 0.64, 0.95 15 0.75 0.53, 0.89 0.10 ‐ 0.15, 0.34

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

High 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 ‐ 0.43, 0.43

Low 20 7 0.35 0.18, 0.57 3 0.15 0.05, 0.36 0.20 ‐ 0.07, 0.44

Low 20 7 0.35 0.18, 0.57 3 0.15 0.05, 0.36 0.20 ‐ 0.07, 0.44

Low 20 5 0.25 0.11, 0.47 3 0.15 0.05, 0.36 0.10 ‐ 0.15, 0.34

Low 20 5 0.25 0.11, 0.47 3 0.15 0.05, 0.36 0.10 ‐ 0.15, 0.34

Low 20 5 0.25 0.11, 0.47 5 0.25 0.11, 0.47 0.00 ‐ 0.26, 0.26

Low 20 5 0.25 0.11, 0.47 5 0.25 0.11, 0.47 0.00 ‐ 0.26, 0.26

r d 95% CI

Table 9: GENE ‐ UP Salmonella vs. Reference Method – POD Results ( INTERNAL ) Matrix GENE ‐ UP Enrichment Inoculum Level N a GENE ‐ UP Salmonella Reference Method X b POD C c 95% CI x POD Raw ground pork BPW 10 hours

Page 26 of 33 b x = Number of positive test portions. c POD C = Candidate method confirmed positive outcomes divided by the total number of trials. d POD r = Reference method confirmed positive outcomes divided by the total number of trials. e dPOD C = Difference between the candidate method presumptive result and candidate method confirmed result POD values. f 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level.

Raw ground pork BPW 24 hours

Raw ground bison BPW 10 hours

Raw ground bison BPW 24 hours Powdered egg BPW

Raw ground pork mTSB 10 hours

Raw ground pork mTSB 24 hours

a N = Number of test portions.