Microbiology Methods for ERP Review 3-2020

DRAFT 1 - AOAC - 05DEC2018

152

GENE-UP ® Salmonella 2 (SLM 2)

050555 - 02 - 2018-12 - en

Method certified NF VALIDATION BIO XX/XX-XX/XX

Matrix

Protocol with an enrichment step at +41.5°C

◦ 25 g of sample. ◦ 225 mL of buffered peptone water (BPW). ◦ Mix using a paddle blender or manually for environmental surface samples. ◦ Incubate at +41.5°C ± 1°C for 18 ‑ 26 hours. Note: For environmental surface samples, add enough BPW to cover the sampling tool (for example 10 mL for a swab, 100 mL for a sponge, 225 mL for cloth). ◦ 25 g of sample. ◦ 225 mL of preheated (+41.5 ± 1°C) buffered peptone water (BPW). ◦ Mix using a paddle blender. ◦ Incubate at +41.5°C ± 1°C for 8 ‑ 24 hours. Protocol for 50–375 g ◦ X g of sample. ◦ 3X mL of preheated (+41.5°C ± 1°C) buffered peptone water (BPW). ◦ Mix using a paddle blender. ◦ Incubate at +41.5°C ± 1°C for 10 ‑ 24 hours. ◦ 25 g of sample. ◦ 225 mL of buffered peptone water (BPW) or specific enrichment broth according to ISO 6579 and 6887. ◦ Mix using a paddle blender for 1 minute or manually for swabs and sponges. ◦ Incubate at +37°C ± 1°C for 18 ‑ 26 hours. Note: For environmental surface samples, add enough BPW to cover the sampling tool (for example 10 mL for a swab, 100 mL for a sponge, 225 mL for cloth). Protocol for 50–375 g ◦ X g of sample. ◦ 9X mL of preheated (+37°C) buffered peptone water (BPW). ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 20 ‑ 28 hours. ◦ Dilute 1/10 in peptone salt or physiological saline before testing. Protocol for 50–375 g ◦ X g of sample. ◦ 9X mL of preheated (+37°C) enrichment broth according to ISO 6579 and ISO 6887. ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 22 ‑ 30 hours. ◦ Dilute 1/10 in peptone salt or physiological saline before testing. Protocol for 50–375 g ◦ X g of sample. ◦ 3X mL of buffered peptone water (BPW) + novobiocin (10 mg/L). ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 20 ‑ 28 hours. Protocol with an enrichment step at +37°C

Standard procedure for all human food products (excluding raw milk products) and production environmental samples (excluding primary production samples)

Raw Meat products (Not Poultry) - Short Enrichment Protocol

Large sample size of Raw Meat Products (Not Poultry)

Method certified NF VALIDATION BIO XX/XX-XX/XX

Matrix

Standard procedure for all human food products (excluding sprouts), pet foods and production environmental samples (excluding primary production samples)

Large sample size of cocoa and chocolate products - Specific protocol

Large sample size of cocoa and chocolate products – ISO enrichment

Large sample size of milk powders with or without probiotics

bioMérieux SA English - 4