Microbiology Methods for ERP Review 3-2020

DRAFT 1 - AOAC - 05DEC2018

153

GENE-UP ® Salmonella 2 (SLM 2)

050555 - 02 - 2018-12 - en

Method certified NF VALIDATION BIO XX/XX-XX/XX

Matrix

Protocol with an enrichment step at +37°C

Protocol for 50–375 g ◦ X g of sample. ◦ 5X mL of preheated buffered peptone water (BPW). ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 20 ‑ 28 hours.

Large sample size of pet foods - Specific protocol

Protocol for 50–375 g ◦ X g of sample. ◦ 9X mL of preheated buffered peptone water (BPW) according to ISO 6579 and ISO 6887. ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 20 ‑ 28 hours.

Large sample size of pet foods - ISO enrichment

Method certified NF VALIDATION BIO XX/XX-XX/XX

Protocol harmonized with GENE ‑ UP ® Cronobacter

Matrix

◦ X g (X mL) of sample. ◦ 9X mL of buffered peptone water (BPW). ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 18 ‑ 26 hours.

Infant formula and infant cereals without probiotics (including ingredients) (up to 25 g)

◦ X g (X mL) of sample. ◦ 9X mL of buffered peptone water (BPW) + novobiocin (10 mg/L). ◦ Mix using a paddle blender. ◦ Incubate at +37°C ± 1°C for 18 ‑ 26 hours.

Infant formula and infant cereals with probiotics (up to 25 g)

AOAC RI Approved Protocols (N°XXXXXX)

Matrix

Protocol

Standard procedure for a 25 g sample for a variety of foods, including fresh raw ground chicken, fresh raw chicken breast, fresh raw fish, creamy peanut butter, vanilla ice cream, and dry pet food

◦ 25 g of sample. ◦ 225 mL of buffered peptone water (BPW) at room temperature (BPW or mTSB for ground chicken). ◦ Homogenize and incubate at +42°C ± 1°C for 18 ‑ 24 hours. ◦ 30 mL of sample. ◦ 30 mL of preheated buffered peptone water (BPW). ◦ Homogenize and incubate at +42°C ± 1°C for 18 ‑ 24 hours. ◦ 375 g of sample. ◦ 1125 mL of preheated buffered peptone water (BPW) or mTSB (only BPW for ground bison). ◦ Homogenize and incubate at +42°C ± 1°C for 10-24 hours. ◦ 375 g of sample. ◦ 1125 mL of preheated buffered peptone water (BPW). ◦ Homogenize and incubate at +42°C ± 1°C for 22 ‑ 26 hours. ◦ 5 enriched samples can also be wet ‑ pooled for analysis (see Standard Procedure 2). Standard Procedure 2 : After incubation, manually mix the contents of the blender bag. Optionally, a sterile technique can be used to remove 1 mL of enriched sample and place it in a pre ‑ labeled microcentrifuge tube. Note: For the wet pooling protocol 375 g Romaine lettuce, combine 1 mL from 5 test sample enrichments in a pre ‑ labeled tube.

Chicken carcass rinse

Beef Trim/Raw Ground Pork/Raw Ground Bison

Romaine lettuce

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