Microbiology Methods for ERP Review 3-2020

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(g) Dynex Technologies 300 µL Sample Tips. (h) Dynex Technologies 1300 µL Reagent Tips. (i) Sample Tubes.—12 mm external diameter and 75 mm height. (j) Polypropylene Tubes.—2 mL and 15 mL and 25 mL. (k) Frit filters.—Optional for matrices with particulate, CAT No. FRI001. (l) 70% v:v Ethanol.—For preparation of the Solus One Salmonella Supplement. (m) Deionized water. (n) Letheen Broth neutralizing buffer. Additional Items Required (a) Incubators – capable of maintaining 37 ± 1ºC and 41.5 ± 1°C (b) Circulating water bath.—capable of maintaining 42–50°C. (c) Heating block or water bath.—capable of maintaining 85–100°C. (d) Autoclave for decontamination of samples. (e) Homogenizer. (f) Measuring cylinder.—capable of measuring 2 L. (g) Vessel or container.—capable of storing 2 L. (h) Filter laboratory blender bags. (i) Multi-channel pipette.—Capable of delivering 100–300 µL. (j) Microplate washer. (k) Microplate reader with 450 nm filter. (l) Calibrated Thermometers.—Capable of measuring a temperature of 37 ± 1°C, 41.5 ± 1°C, and 85–100°C. (m) Adjustable, variable volume pipettes capable of sampling and delivering 10-1000 µL. (n) Micropipette tips – Aerosol resistant. (o) Serological Pipette Bulbs (Automatic Pipette) – For sampling and delivering of 1 mL - 10 mL (p) Serological pipettes – Aerosol resistant (q) Refrigerator – Capable of maintaining 2-8 °C (r) Vortex Mixer (s) Calibrated Timer. (t) Marker. C. General Instructions (a) Store the Solus One Salmonella test kit at 2-8°C. Do not freeze. Keep kit away from light during storage. After opening, unused reagent tubes should always be stored at 2-8°C. Do not use the Solus One Salmonella test kit past the expiration date.

(b) Follow all instructions carefully. Failure to do so may lead to inaccurate results.

D. Safety Precautions

Solus One Salmonella

Ensure that the heating block reaches a temperature of 85–100°C and the sample is heated for 15–20 min thus ensuring the organisms are killed and the sample is safe to

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