Microbiology Methods for ERP Review 3-2020

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E. Test Portion Analysis (a) Solus One

1) When incubation is complete, carefully remove a 1 mL aliquot, avoiding particulate and fatty matter. Heat aliquot to 85–100°C for 15–20 min. Allow to cool to room temperature (18–25°C), or place test tubes in cold water for 5 min. Keep the unheated samples for confirmation. Samples can be stored up to 72 h at 2–8°C. 2) Prior to analysis, bring the entire contents of the kit to room temperature (15–25°C) by placing the kit on a sanitized bench top for 1 h prior to use. 3) Determine the number of wells required for the test. Take the required number of strips from the pouch and fit them to the frame provided. Note: Unused strips should be returned to the pouch and stored at 2–8°C. 4) Dynex DS2 Automated procedure : Turn on power to the Dynex DS2 instrument, followed by the power to the computer. Open the Matrix software on the computer and enter login information. The Dynex DS2 instrument will perform a self- diagnostic check. In the DS- Matrix software, select “File”, “Worklist Editor”. Then select “New Sample Batch”. a. Enter your sample batch ID as required. Enter the total number of samples in the batch. Sample ID may then be typed into the empty positions in the rack or assigned automatically using an onboard barcode scanner. b. If using an onboard barcode scanner slide the sample tube racks onto the machine and click next. c. Click on the Solus One Salmonella assay. Then click the “Add Assay” button. d. Select “Done” when assay selection is completed. e. Select the “Accept” button to start the run. f. After the timeline has been accepted, loading of sample tube racks, reagents and consumables is initiated.

g. If not already onboard, load sample test tube racks into the slots.

h. Remove the lid from the pipette boxes and load into the instrument. i. Load the Conjugate, TMB Substrate, and Stop Solution into the correct locations specified by the instrument.

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