Microbiology Methods for ERP Review 3-2020

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(b) Comply with Good Laboratory Practices. (c) Do not use reagents after the expiration date indicated on the label. (d) Visually inspect vials before testing. Do not use vials with evidence of damage, leakage, or deterioration. (e) Do not mix reagents (or disposables) from different lots. (f) Powder-free latex or nitrile gloves are recommended for all PCR steps. (g) The equipment and accessories should be regularly cleaned and decontaminated. (h) Never remove the caps from the lysis tubes. (i) Do not try to remove the strip tube caps once they have been sealed to the PCR strip tubes (j) Spills should be wiped up thoroughly after treatment with bleach or a nucleic acid degradation solution. See the GENE-UP ® user manual for information on cleaning spills on or in the instrument. Do not autoclave solutions containing bleach. Dispose of used or unused reagents as well as any other contaminated disposable materials following procedures for infectious or potentially infectious products. It is the responsibility of each laboratory to handle waste and effluents produced according to their type and degree of hazardousness and to treat and dispose of them (or have them treated and disposed of) in accordance with any applicable regulations. Salmonella is a Gram-negative facultative rod-shaped bacterium organism. Care must be taken when handling samples that may contain Salmonella . Strict compliance with BSL-2 practices, containment equipment, and facilities are recommended for all activities utilizing known or potentially infectious clinical materials or Safety Precautions

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