Microbiology Methods for ERP Review 3-2020

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acid. Store at 2–8°C.

( g ) Washing Buffer Concentrate (25x) .— 6 x 10 mL or 5 x 60 mL. Store at 2–8°C. ( h ) Washing Buffer Activator .— 6 x 1 Sachet or 5 x 1 Sachet. Store at 2–8°C.

( i )

Product Instructions .

D. Additional supplies and reagents

( a ) Dehydrated Buffered Peptone Water (BPW) Medium according to ISO 6579, CAT No. MED017 . 8 ( b ) Dehydrated Solus modified Buffered Peptone Water (Solus mBPW), CAT No. MED038 . 9 ( c ) Solus One Salmonella Supplement , CAT No. SALSUPP ‐ 22.5 & SALSUPP ‐ 112 . 10 ( d ) Dynex Technologies DS2 Instrument (Dynex DS2) . — Dynex Technologies, Chantilly, VA. 11 ( e ) Computer with DS2 Software . 12 ( f ) Dynex Technologies 300 µL Sample Tips . 13 ( g ) Dynex Technologies 1300 µL Reagent Tips . 14 ( h ) Sample Tubes .— 12 mm external diameter and 75 mm height. 15 ( i ) Polypropylene Tubes .— 2 mL and 15 mL and 25 mL. 16 ( j ) Frit filters .— Optional for matrices with a lot of particulate , CAT No. FRI001. 17 ( k ) 70% v:v Ethanol .— For preparation of the Solus One Salmonella Supplement. 18 ( l ) Deionized water . 19 ( m ) Sterile collection sponge for sampling environmental surfaces .— World Bioproducts EZ Reach 20 Sponge Sampler with Hi Cap Neutralizing Buffer, or equivalent. 21 ( n ) Sterile collection swab for sample environmental surfaces . 22 ( o ) Letheen Broth neutralizing buffer .

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E. General Preparation

( a ) Use aseptic techniques.

( b ) Use filter laboratory bags during enrichment to minimize particulates.

( c ) Separate work areas for the following: media preparation, sample preparation, and pathogen

detection.

( d ) Clean the work stations with a disinfectant of choice before and after use. (Sodium

31 hypochlorite solution, phenol solution, Quaternary ammonium solution, etc.) 32 ( e ) Do not reuse kit disposables. 33 ( g ) Change pipette tips in between samples.

Pathogen Detection (Manual Method)

( a ) Equilibrate test kit to room temperature (15–25°C) an hour before use.

( b ) Ensure that the bench processing time of inoculated samples is kept to a minimum, to avoid

extensive growth of competing non ‐ Salmonella bacteria.

( c ) Start heating block or heat water bath to 85–100°C prior to initiating testing.

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