Microbiology Methods for ERP Review 3-2020

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in the GENE-UP ® Lysis Tube Holder. If less than 8 tubes in a strip are required, the strips can be cut apart. Note: Never open the lysis tubes. If a lysis tube opens or leaks, this should be considered a contamination event. (c) Clip the GENE‑UP ® Lysis Tube Holder on the GENE‑UP ® Heavy Rack Holder. (d) Transfer 20 μL of sample into the lysis tube. Use the Plate Map from the GENE‑UP ® Routine software to pipet each sample into the correct plate position. (e) Remove the GENE‑UP ® Lysis Tube Holder from the GENE‑UP ® Heavy Rack Holder. (f) Clip the GENE‑UP ® Lysis Tube Holder on the Troemner Vortex Mixer Adaptor. (g) Run the vortex mixer at 2200 rpm for 5 min. Note: When using the Vortex‑Genie ® Pulse, fit it with the GENE‑UP ® Lysis Rack Adaptor. Run the vortex at maximum speed for 5 min. The maximum speed must be above 2000 rpm. (h) When lysis is complete, remove the GENE‑UP ® Lysis Tube Holder from the Troemner Vortex Mixer Adaptor. (i) Clip the GENE‑UP ® Lysis Tube Holder into the GENE‑UP ® Heavy Rack Holder and proceed to Final Setup for PCR. Note: The lysate can be stored for up to 3 days at 2–8°C or at -15 to -31°C for extended storage.

F. PCR Preparation

Before beginning the procedure, don a clean pair of powder-free latex or nitrile gloves. (a) Use the plate map created in the GENE‑UP ® Routine software to determine the number of PCR tubes required from the GENE‑UP ® PCR Kit and place the correct number of PCR tubes in the GENE‑UP ® PCR Tube Holder. If less than eight tubes in a strip are required, the strips can

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