Microbiology Methods for ERP Review 3-2020

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GENE‑UP ® Lysis Tube Remover Tool. The GENE‑UP ® Lysis Tube Holder is reusable, but the used lysis tubes should be disposed of accordingly. (i) Please refer to the appropriate GENE‑UP ® instrument user manual for instructions to start a run, view results, and use the GENE‑UP ® Routine software.

G. Results and Interpretation

(a) Results are automatically interpreted once the PCR run is completed. The routine software interprets data for each sample and gives a positive, negative, or inhibited result as indicated in the following table

Salmonella spp. (640 nm)

Internal amplification Control (705 nm)

Result

+ +

+

+ +

-

- -

+

-

! Inhibition

-

H. PCR Inhibition Protocol (a) In case of an inhibited result, dilute the lysate to 1:3 in the control buffer: (b) Transfer 10 μL of control buffer in an adapted microtube. (c) Follow the same procedure in the PCR PREPARATION section of this document, using 10 μL of this dilution of lysate. Note: It is recommended to retest in parallel the lysate without dilution. Note: In case of inhibited results at 1:3, you can dilute the lysate to 1:10. Note: Some matrices like aromatics herbs or cocoa powders may contain inhibitory molecules. For these matrices, 1 mL of enriched sample could be diluted 1:5 or 1:10 in Tryptone

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