Microbiology Methods for ERP Review 3-2020

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(b) Use a loop to isolate the sample directly from the second enrichment. (c) Streak the sample on SALSA, ASAP, CHROMID Salmonella and XLD agar plates. (d) Incubate at 35°C ± 1°C for 24 ± 3 h. (e) Alternatively, VIDAS ® SLM (or SPT) can be performed with an input volume of 500 μL of SX2 broth. (f) If no typical Salmonella colony is identified, the result is considered negative. (g) If a typical colony is obtained, test an isolated colony directly using a Salmonella spp. latex, API 20E strip, VITEK GN or VITEK MS. External quality control can be performed using one Salmonella strain. (a) Add one isolated colony from a fresh and pure culture in 9 mL of Buffer Peptone Water. (b) Mix and incubate at 42 ± 1°C for 18‑24 h. (c) Dilute 1/100 of the culture in BPW in order to obtain a suspension containing approximately 10 6 cells/mL of the strain. (d) Follow the protocol from the SAMPLE LYSIS section steps to CONFIRMATION OF POSITIVE RESULTS sections. (e) Check that the results obtained correspond to the characteristics of the tested strains. J. Quality Control

K. Limitations of the Method

The GENE‑UP ® Salmonella (SLM) kit has been evaluated on a large number of matrices. However, given the wide variety of products and manufacturing procedures, it is recommended to check that the composition of the matrices tested does not affect the reliability of GENE‑UP ®

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