Microbiology Methods for ERP Review 3-2020

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Table 2020.2A (cont’d.): Comparative results for the detection of Salmonella in raw ground beef (80% lean) test portions by the GENE-UP ® Salmonella 1 (SLM) method with alternative confirmation vs. MLG 4.10 reference method in a collaborative study (high inoculum level) 2 Statistic Matrix Laboratory Candidate presumptive (CP) Candidate confirmed (CC) Candidate result (C) Reference method (R) C vs. R CP vs. CC N X POD (CP) N X POD (CC) N X POD (C) N X POD (R) dLPOD (C,R) dLPOD (CP,CC) Raw ground beef (80% lean) 1 a 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 2 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 3 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 4 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 5 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 6 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 7 12 12 1.00 12 11 0.92 12 11 0.92 12 8 0.67 0.25 0.08 8 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 9 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 10 a 12 11 0.92 12 10 0.83 12 10 0.83 12 2 0.17 0.66 0.09 MPN/ Test Portion 3.43 2.72 4.88 11 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 12 12 12 1.00 12 12 1.00 12 12 1.00 12 12 1.00 0.00 0.00 13 12 12 1.00 12 11 0.92 12 11 0.92 12 11 0.92 0.00 0.08 Estimate All 132 132 1.00 132 130 0.98 132 130 0.98 132 127 0.96 0.02 0.02 LCL 0.95 0.95 0.95 0.89 -0.03 -0.01 UCL 1.00 1.00 1.00 1.00 0.09 0.04 s r b 0.12 0.00 s L c 0.00 0.00 s R d 0.12 0.00 SE e N/A 0.01 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e Standard Error 3

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit 4 5 6 7 8