Microbiology Methods for ERP Review 3-2020

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Table 2020.2B: Comparative results for the detection of Salmonella in raw ground beef (80% lean) test portions by the GENE-UP ® Salmonella (SLM) 1 method with traditional confirmation vs. MLG 4.10 reference method in a collaborative study (non-inoculated control) 2 Statistic Matrix Laboratory Candidate presumptive (CP) Candidate confirmed (CC) Candidate result (C) Reference method (R) C vs. R CP vs. CC N X POD (CP) N X POD (CC) N X POD (C) N X POD (R) dLPOD (C,R) dLPOD (CP,CC) Raw ground beef (80% lean) 1 a 12 0 0.00 12 2 0.17 12 0 0.00 12 0 0.00 0.00 -0.17 2 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 3 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 4 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 5 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 6 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 7 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 8 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 9 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 10 a 12 1 0.08 12 0 0.00 12 0 0.00 12 1 0.08 -0.08 0.08 11 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 12 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 13 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 0.00 0.00 Estimate All 132 0 0.00 132 0 0.00 132 0 0.00 132 0 0.00 0.00 0.00 LCL 0.00 0.00 0.00 0.00 -0.03 -0.02 UCL 0.03 0.03 0.03 0.03 0.03 0.02 s r b 0.00 0.00 s L c 0.00 0.00 s R d 0.00 0.00 SE e N/A 0.01 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e Standard Error 3

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit 4 5 6