Microbiology Methods for ERP Review 3-2020

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Table 2020.2B (cont’d.): Comparative results for the detection of Salmonella in raw ground beef (80% lean) test portions by the GENE-UP ® Salmonella 1 (SLM) method with traditional confirmation vs. MLG 4.10 reference method in a collaborative study (low inoculum level) 2 Statistic Matrix Laboratory Candidate presumptive (CP) Candidate confirmed (CC) Candidate result (C) Reference method (R) C vs. R CP vs. CC N X POD (CP) N X POD (CC) N X POD (C) N X POD (R) dLPOD (C,R) dLPOD (CP,CC) Raw ground beef (80% lean) 1 a 12 4 0.33 12 5 0.42 12 3 0.25 12 9 0.75 -0.50 -0.09 2 12 5 0.42 12 4 0.33 12 4 0.33 12 8 0.67 -0.34 0.09 3 12 5 0.42 12 5 0.42 12 5 0.42 12 6 0.50 -0.08 0.00 4 12 6 0.50 12 7 0.58 12 6 0.50 12 3 0.25 0.25 -0.08 5 12 5 0.42 12 6 0.50 12 5 0.42 12 5 0.42 0.00 -0.08 6 12 4 0.33 12 4 0.33 12 4 0.33 12 4 0.33 0.00 0.00 7 12 4 0.33 12 4 0.33 12 4 0.33 12 2 0.17 0.16 0.00 8 12 3 0.25 12 3 0.25 12 3 0.25 12 6 0.50 -0.25 0.00 9 12 5 0.42 12 5 0.42 12 5 0.42 12 4 0.33 0.09 0.00 10 a 12 3 0.25 12 4 0.33 12 1 0.08 12 0 0.00 0.08 0.17 MPN/ Test Portion 0.49 0.37 0.63 11 12 4 0.33 12 4 0.33 12 4 0.33 12 3 0.25 0.08 0.00 12 12 6 0.50 12 6 0.50 12 6 0.50 12 8 0.67 -0.17 0.00 13 12 4 0.33 12 3 0.25 12 3 0.25 12 2 0.17 0.08 0.08 Estimate All 132 51 0.39 132 51 0.39 132 49 0.37 132 51 0.39 -0.02 0.00 LCL 0.33 0.31 0.31 0.27 -0.15 -0.04 UCL 0.44 0.46 0.43 0.51 0.12 0.04 s r b 0.50 0.50 s L c 0.00 0.00 s R d 0.50 0.50 SE e N/A 0.02 a Collaborator Data Not Included in Statistical Analysis, b Repeatability Standard Deviation, c Among-Laboratory Standard Deviation, d Reproducibility Standard Deviation, e Standard Error 3

N - Number of Test Portions; X – Number of Positive Test Portions; LCL – Lower Confidence Limit; UCL – Upper Confidence Limit 4 5 6