Microbiology Methods for ERP Review 3-2020

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GENE ‐ UP ® SLM 2 Collaborative Study

July 2019

Appendix 8.3  ‐ Study Materials

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Materials provided by collaborator a) Sterile pipette tips – Capable of 20µL

4 b) Multi ‐ channel pipette . – Capable of 20µL and 10 µL 5 c) Serological pipette bulbs and serological pipettes – For sampling and delivering of 0.1  ‐ 10 mL 6 d) SMASHER ® XL . – bioMérieux or equivalent 7 e) Type P filter bag. ― bioMérieux or equivalent 8 f) Thermometer . – Calibrated range to include 100 ± 1°C range 9 g) Incubators . – Capable of maintaining 42 ± 1°C and 35 ± 1°C 10 h) Advanced Digital Heavy Duty Vortex Mixer. 11 i) GENE ‐ UP® Lysis Rack Adaptor 12 j) Plate Centrifuge  ‐ MPS1000 Mini PCR Plate Spinner 13 k) GENE ‐ UP® PCR Tube Holder 14 l) GENE ‐ UP® Lysis Tube Remover 15 m) GENE ‐ UP® Heavy Rack Holder 16 n) GENE ‐ UP® Lysis Tube Holder 17 o) Refrigerator . – capable of maintaining 2 ‐ 8°C

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Materials and reagents provided by the study sponsor a) GENE ‐ UP Salmonella 2 Test Kits – Quantity 1

b) GENE ‐ UP Lysis Test Kits – Quantity 1

c) BPW (4 x 3L) – Quantity 4

d) 10 µL Biotix filter pipette tip   ‐ Quantity 1 case

e) Modified tryptic soy broth f) Tetrathionate broth

g) Modified Rappaport Vassiliadis medium h) Brilliant green sulfa agar (BGS) i) Double modified lysine iron agar (DMLIA)

j) ASAP agar

k) CHROMID Salmonella l) Triple sugar iron agar (TSI) m) Lysine iron agar (LIA)

n) Tryptic soy agar with 5% Sheep Blood (TSAB) o) Polyvalent somatic O antisera – 1 box p) Polyvalent flagellar H antisera – 1 box 36 q) API 20 E or VITEK GN Cards – Quantity 2 x 25 (API 20E) or 3 x 20 (VITEK GN) 37 r) API 20 E Reagents 38 a. James Reagent – Quantity 2 x 1 ampule 39 b. VIP1/VIP2– Quantity 1 box 40 c. TDA– Quantity 1 box 41 d. NIT1/NIT2 – Quantity 1 box 42

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