Microbiology-PTM-OMA_Modules_1-5

Testing Requirements • For qualitative methods:

• Grow inclusivity organisms according to all candidate method enrichment protocols. If the  only difference is time of enrichment, follow the shortest enrichment time. Dilute, if  necessary, to 100x LOD. • Grow exclusivity organisms under appropriate conditions (broth(s)/temperature) to  encourage growth.  Test at highest growth level achieved. • For quantitative methods: • Grow all organisms under appropriate conditions (broth(s)/temperature) to encourage  growth.  • Dilute inclusivity organisms to 100x LOD. • Test exclusivity organisms at the highest growth level achieved. • For ID Methods: • Streak inclusivity and exclusivity organisms to all claimed agars and/or dilute in all claimed  buffers. • Also include one non‐selective agar, if not already included in claim.