Microbiology-PTM-OMA_Modules_1-5
Performing the Study – Test Portions
• From each material, remove the replicate test portions needed for the Candidate and Reference Methods • Artificially or naturally contaminated matrix • Paired or unpaired analyses • Qualitative or Quantitative method comparison • Randomize and blind code the test portions for each method • From Materials 2 and 3, remove replicate test portions needed for Most Probable Number (MPN) determination – Qualitative methods only • Not required for noninoculated material • Need at least 5 replicates at 3 levels for MPN • Reference method only • Can use the reference method replicates from the matrix study as one level of the MPN
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