Microbiology-PTM-OMA_Modules_1-5

Replicates for Composite Test Portions

• Inoculate materials at high and low levels • Low level must achieve fractional positive results for 25‐g test portions • For the reference method, analyze 25‐g test portions of all materials • For the candidate method, mimic composite by combining one 25‐g  test portion from a test material with 300 g noninoculated matrix (for  a 325 g composite) or 350 g noninoculated matrix (for a 375‐g  composite)