Microbiology-PTM-OMA_Modules_1-5

Analyzing Data – Qualitative Methods

• Use Probability of Detection (POD) statistics • POD is the proportion of positive analytical outcomes for a qualitative method for a given  matrix at a given bacterial level or concentration • POD = x/N • Unblind the data • Analyze each level of each matrix separately • Analyze data separately if there are multiple time points, multiple instruments,  manual and automated methods, etc. • Calculate POD and 95% confidence interval (CI) for each method • POD CP for the candidate method presumptive results • POD CC for the candidate method confirmation results • POD C for the candidate method final results (accounting for presumptive and confirmatory  results) • POD R for the reference method results