Microbiology-PTM-OMA_Modules_1-5

Data Table – Qualitative Method SLV

Table 1. Comparison of presumptive and confirmation candidate method results

Presumptive

Confirmation

MPN b / test portion N a

Matrix (APC i )

Strain

Instrument

dPOD CP f

95% CI g

POD CP d (95% CI g ) 0.00 (0.00, 0.43) 0.00 (0.00, 0.43) 0.80 (0.58, 0.92) 0.80 (0.58, 0.92) 1.00 (0.57, 1.00) 1.00 (0.57, 1.00)

POD CC e (95% CI g ) 0.00 (0.00, 0.43) 0.00 (0.00, 0.43) 0.80 (0.58, 0.92) 0.80 (0.58, 0.92) 1.00 (0.57, 1.00) 1.00 (0.57, 1.00)

X c

X c

A

0

0

0.00 (‐0.47, 0.47)

N/A

5

B

0

0

0.00 (‐0.47, 0.47)

Smoked  Salmon (7.0 x 10 5 CFU/g)

A

16

16

0.00 (‐0.13, 0.13)

L.  monocytogenes  ATCC 15313

0.91 (0.64, 1.76)

20

B

16

16

0.00 (‐0.13, 0.13)

A

5

5

0.00 (‐0.47, 0.47)

7.34 (3.65, 16.76)

5

B 0.00 (‐0.47, 0.47) a N = Number of test potions. b MPN = Most Probable Number with 95% confidence interval.  c x = Number of positive test portions.  d POD CP =  probability of detection for the candidate presumptive results.  e POD CC = probability of detection for the candidate confirmed results.  f dPOD CP =  POD CP minus POD CC .  g 95% Confidence Interval.  i APC = Aerobic Plate Count.  5 5

Note : Comparison of presumptive and confirmation results is always a paired comparison.

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