Microsoft Word - Candidates for 2018 ERP of the Year

77

Protein:   Method Reviews and Decisions

AOAC  Method # 

Manuscript Title, Submitter and Reviewers 

RP Decisions / Consensus 

Title:   Identification of Pea, Rice and Soy Proteins  in Raw Materials and Finished Goods using ESI  HPLC‐MS/MS  Author(s):  (METHOD AUTHOR’S NAME), Genysis  Labs  Submitted by: Aihua Liu, Genysis Labs Primary Reviewer:   Sneh Bhandari, Mérieux  NutriSciences

MOTION to move this method to First Action Official Methods of Analysis ® Status  (You/Zielinski)   6 in favor, 2 opposed, 1 abstention.  The motion failed.  Reasoning/Summary:   Dissenters gave their reasoning.  Szpylka stated that the method works but he would like to see  the proof of the uniqueness of the three peptides that were chosen.  Yang stated that he is not  happy with the specificity of the method.  Also, in the method’s Table 2, some of the molecules  are not relevant to this method.    Schaneberg explained that some of these issues can be addressed even after the method  moves forward; prior to Final Action status.    MOTION to move this method to First Action Official Methods of Analysis ® Status  (Phinney/Zielinski)  7 in favor, 2 opposed, no abstentions.  The motion passed by 2/3 override.   Description of the process used to determine the applicability of the two or three  peptides, specifically identifying the sources of the non‐animal derived proteins.   Clarify the columns used in the method.   Clarify the trypsin source and the digestion optimization data.   Clarify concentration of urea in method.   Formulation details on Matrix A and Matrix B.   Provide a statement of orthogonal protein identity.    Reproducibility and user feedback  MOTION to approve the Final Action Official Methods of Analysis ® Status Requirements as  presented (Szpylka/Rimmer)  8 in favor, 0 opposed, 1 abstention.  The motion passed. Final Action Requirements: 

               PRO‐001

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03/12/2018