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STAKEHOLDER PANEL ON STRATEGIC FOOD ANALYTICAL METHODS EXPERT REVIEW PANEL ON ETHANOL IN KOMBUCHA

METHODS FOR CONSIDERATION: Conclusion: The Expert Review Panels reviewed five (5) Kombucha methods, which were submitted in response to the Call for Methods. Methods Reviewed: Each method collected by AOAC for consideration by this ERP was assigned a set of reviewers. Methods reviewed included: • KOM-04: Determination of ethanol in Kombucha by Gas Chromatography-Flame Ionization Detector: Intra-Laboratory Validation o Resubmission o Author(s): Xin Du and Yonglin Ren o Submitted by: Xin Du • KOM-05: Determination of Alcohol Content in Kombucha Tea by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry o Resubmission o Author(s): Katherine K. Stenerson o Submitted by: Katherine Stenerson, Millipore Sigma •

KOM-06: Enzymic method for measurement of Ethanol in Kombucha o Author(s): R. Ivory, E. Delaney, J. Larkin, V. McKie and B.V. McCleary o Submitted by: Ruth Ivory, Megazyme • KOM-07: Enzytec ™ Liquid Ethanol o Author(s): Markus Lacorn and Thomas Hektor o Submitted by: Patricia Meinhardt, R-Biopharm, Inc. • KOM-08: AP-Chrom-GCMS-Kombucha-ABV o Author(s): Michael J. Goodrich o Submitted by: Michael Goodrich, Cornerstone Labs

All methods were reviewed against AOAC SMPR 2016.001, Standard Method Performance Requirements (SMPRs®) for Determination of Ethanol in Kombucha. The decisions of the September 24, 2017 ERP are shown below.

03/12/2018