Microsoft Word - Candidates for 2018 ERP of the Year

267

Cons/Weaknesses of the Manuscript: ER 1

Some editorial suggestions are made below.

ER 2 ER 3

NA

Need to elaborate on the issues and possible causes of those issues of laboratories whose data were not used in the study. • Correction on table 2 page 17, AOAC OMA 993.12, lab 6 is excluded in the analysis and should have a superscript b. • It is unclear as to why the reference method chosen is not the BAM reference method which would be in this reviewer’s opinion a better and more comprehensive method to use. While the reference method used is an OMA method, the use of the BAM method would have given more confidence to regulatory bodies reviewing this method. • The uses of positive and negative controls are not well defined. • There may be some value in stating the ISO status of the testing labs to add more confidence on the results. • The reasons for the exclusion of labs 6 and 13 may need more detail. In both cases the data set suggests that these labs were simply excluded since they detected false negative results which I am sure is not the case; however, no detail scientific explanation is provided. • While many of the acronyms used in the data and statistical tables are well known and are described in the Appendix J of the AOAC method validation guidelines, it may be beneficial to include a section on the explanation of these terms. Since this is my first time reviewing such a manuscript, I am not sure if these were included in the past but they may help some understand the tables. The manuscript does not discuss the reasons behind the selection of DF broth enrichment protocol for the collaborative study: mLRB vs DF? 1 step enrichment vs 2 step enrichment? There are very few mistakes in the report among which one is more relevant: table 2 presenting individual collaborator results seem wrong for the low level test portion results as the total from the table give 67 positive when the report mentions 73. Comparing data with table 2014.2A (p21), it seems that numbers for labs 2 and 8 are wrong in table 2: is that right? Please advise.

ER 4

ER 5

ER 6 ER 7 ER 8

NO

None observed.

None Supporting Data and Information: Does data from collaborative study support the method as written? ER 1 Yes. ER 2 Yes ER 3 Yes ER 4

Since the cottage cheese is a posturized product, a heat stress inoculum would have been more appropriate; however, this was also not done during the pre-collaborative study. Yes & No. Yes when it relates to the DF-1 step enrichment protocol. No because mLRB and DF-2 step enrichment protocols have not been evaluated during this collaborative study. That is my understanding that each of the different enrichment protocols must be evaluated during a collaborative study to be submitted to OMA first action: am I mistaken? Some of the claimed matrices require a 2-step enrichment protocol (bagged raw spinach and whole cantaloupe) I marked "no" for questions 3 - 6 above, because Tables A and B make "claims" that are not supported by the PTM or collaborative studies. Both tables have a section for "other matrices" that include dairy, vegetables, meat, poultry, seafood, and fruit, but none of these categories have been fully validated (only 1 or 2 matrices per category; traditionally, you need at least 3 matrices to claim a category). Please remove the "For Other Matrices" sections from both Tables A and B, so that the Tables will be aligned yes

ER 5

ER 6 ER 7

03/12/2018