Microsoft Word - Candidates for 2018 ERP of the Year

275

General comments about the method: ER 1 None. ER 2

The inter-laboratory reproducibility of the method is evaluated in two foods, cottage cheese and deli turkey. Heat stressed cells of L. monocytogenes were used for the latter matrix. Page 3, row 27-28, Remove shipped to collaborators as it is described at page 4 row 1 along with randomizing and blind coding. The method appears to be a very rapid method with a simple work flow that allows for the rapid detection of Listeria monocytogenes. The method is very well described in the various documents , and the various steps of the sample preparation and MDA assay are exhaustively presented, including the ciritical steps. The method can be applied to a large variety of matrices covering different food categories and environmental surfaces. Enrichment protocol may vary according to the matrix, and therefore, users shall be clearly informed about the enrichment conditions for given matrices. Enrichment times usually mention a upper limit of incubation: what does happen if the user exceeds this upper value? This is a method very useful for the industry and it allows more short times for detection of Listeria than with culture methods . So, this is very useful for facilitate regional trade. No additional comments

ER 3

ER 4 ER 5

ER 6

ER 7

Well conceived and scientifically sound

ER 8

Pros/Strengths of the Manuscript: ER 1 Well written. ER 2

The validation is generally clearly described.

Very well written.

ER 3 ER 4 ER 5 ER 6

Generally well written.

Manuscript is well written and the information flow is in an understandable order. Exhaustive presentation of the various AOAC-PTM validations/extensions. Complete description of the sample preparation protocols. Efficient description of the collab study workflow and organization. Tables are very useful for summarizing the various enrichment protocols of the method, for presenting the results.

The method has a high sensitivity and specificity.

ER 7 ER 8

Clearly written and explained

03/12/2018