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AOAC RESEARCH INSTITUTE  Expert Review Panel Chair Report for Veterinary Drug Residues – Dyes in Seafood   Page 3 of 6 

EXPERT REVIEW PANEL, METHOD BACKGROUND, AND CONCLUSIONS 

Criteria for Vetting Methods to be considered:  AOAC convened the Official Methods of Analysis SM  (OMA) Expert Review Panel (ERP) for Veterinary Drug  Residues ‐ Dyes in Seafood, that was held via teleconference and Adobe web connect on Monday, December 7,  2015 at 11:00am Eastern Standard Time (EST).   The purpose of the meeting was to review and evaluate AOAC 2012.25:  Three Triphenylmethane Dyes Residues  and Their Metabolites (Malachite Green, Leuco Malachite Green, Crystal Violet, Leuco Crystal Violet, and  Brilliant Green) in Aquaculture Product, LC‐MS/MS, First Action 2012, for recommendation of AOAC Final Action  Official Methods status.  Supplemental information was provided in an e‐book 1  to the reviewers which included  the following documents:  3) AOAC 2012.25:  Three Triphenylmethane Dyes Residues And Their Metabolites (Malachite Green, Leuco  Malachite Green, Crystal Violet, Leuco Crystal Violet, Brilliant Green) In Aquaculture Product, LC‐MS/MS,  First Action 2012  4) an Article: The Monitoring of Triphenylmethane Dyes in Aquaculture Products Through the European  Union Network of Official Control Laboratories  5) Method Feedback – Wendy Andersen, U.S. Food and Drug Administration,   6) Article: Expansion of the Scope Of AOAC First Action Method 2012.25—Single‐Laboratory Validation of  Triphenylmethane Dye and Leuco Metabolite Analysis in Shrimp, Tilapia, Catfish, and Salmon by LC‐ MS/MS  7)  Article: Determination Of Triphenylmethane Dyes And Their Metabolites In Salmon, Catfish, and Shrimp  1) Collaborative Study Manuscript   2) AOAC Expert Review Panel Report (dated May 7, 2013) 

by LC‐MS/MS Using AOAC First Action Method 2012.25: Collaborative Study  8) Method Feedback – Robert Burger, U.S. Food And Drug Administration  9) Method Feedback – Yanxuan Cai, U.S. Food And Drug Administration  10) Method Feedback – Ross Potter, Canadian Food Inspection Agency  11)  Method Feedback – Steven Lehotay, United States Department Of Agriculture 

Criteria for Vetting Experts and Selection Process:  The current Expert Review Panel (ERP) consisting of ten (10) experts that were submitted for consideration by  the Official Methods Board to evaluate candidate methods for Veterinary Drug Residues  ‐ Dyes in Seafood as  per the Expert Review Panel (ERP) Policies and Procedures.  The experts are Joe Boison (Chair), Haejung An,  Martin Danaher, Doug Hite, Brian Kinsella, Perry Martos, Katerina Mastovska, Cory Murphy, Jon Reuther, and  Sherry Turnipseed.   ERP Orientation:   All ERP members have completed the mandatory AOAC Expert Review Panel Orientation Webinar and/or have  participated in previous AOAC Expert Review Panel meetings.  

Expert Review Panel Meeting Quorum 

1  Expert Review Panel E‐Book ‐ http://griegler‐aoac‐org.cld.bz/AOAC‐RI‐ERP‐Book‐VDR

03/12/2018