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AOAC RESEARCH INSTITUTE  Expert Review Panel Chair Report for Veterinary Drug Residues – Dyes in Seafood   Page 4 of 6 

The meeting of the Expert Review Panel was held via teleconference and Adobe Web Connect. A quorum is the  presence of seven (7) members or 2/3 of the total vetted ERP, whichever is greater.  Seven (7) members out of  the ten (10) voting members were present and met a quorum to conduct the meeting.  Standard Method Performance Requirements (SMPRs):   The collaborative study was reviewed against the attached AOAC SMPR 2009.001: Standard Method  Performance Requirements for Quantitative Methods for Drug Residues in Shrimp, Tilapia, Catfish, and Salmon  that was approved by the AOAC Stakeholder Panel on Marine and Freshwater Foods on March 31, 2010.  Conclusion:   The ERP reviewed AOAC 2012.25:  Three Triphenylmethane Dyes Residues And Their Metabolites (Malachite  Green, Leuco Malachite Green, Crystal Violet, Leuco Crystal Violet, Brilliant Green) In Aquaculture Product, LC‐ MS/MS, First Action 2012 against the AOAC SMPR 2009.001:Standard Method Performance Requirements for  Quantitative Methods for Drug Residues in Shrimp, Tilapia, Catfish, and Salmon. The ERP also reviewed the  method against the optional and required items noted for consideration of AOAC Final Action status from the  AOAC Expert Review Panel report of May 7, 2013.    

1) Select a 3rd confirmation ion (optional).  2) Add a 5th non‐zero calibration point (optional).  3) Clarify shelf life for the standard and provide data to support.  4) Edit typo in the title of the draft First Action paper. 

The ERP concluded that AOAC 2012.25 should be recommended for AOAC Final Action Official Methods status  by consensus as noted in the meeting minutes.  Subsequent ERP Activities:   The ERP will draft a recommendation report for AOAC 2012.25 and submit the completed report to the AOAC  Official Methods Board for AOAC Final Action Official Methods status.  

03/12/2018