Microsoft Word - Draft OMB Meeting Agenda-April 11 2019

OMB Meeting 4-11-2019 Pre-read Materials

27

TN-1208

Figure 2. Cobalamin Mobile Phase pH Selection Chart

2.22

Mobile phase at pH

3.5

1.2

Charge

0.18

-0.84

-1.86

8

10

12

14

0

2

4

6

pH

Figure 3. Tuning files _ Methylcobalamin (m.w. 1343.59) by infusion

[M+2H + ]

673.0

7.4e7 7.0e7 6.5e7 6.0e7 5.5e7 5.0e7 4.5e7 4.0e7 3.5e7 3.0e7 2.5e7 2.0e7 1.5e7 1.0e7 5.0e6

Methylcobalamin (MeB 12 )

684.0

695.0

0.0 Intensity,cps Intensity,cps Intensity, cps 500 1.4e7 1.3e7 1.2e7 1.1e7 1.0e7 9.0e6 8.0e6 7.0e6 6.0e6 5.0e6 4.0e6 3.0e6 2.0e6 1.0e6 147.1 0.0 100 1.27e6 1.20e6 1.10e6 1.00e6 9.00e5 8.00e5 7.00e5 6.00e5 5.00e5 4.00e5 3.00e5 2.00e5

[M-ME+2H + ]

665.5 687.5 674.5 762.9 622.9 700.0 774.0

797.0

831.0

527.0 541.0

882.8 989.51004.5

1366.6

609.2

App ID 23914

600

700

800

900

1000

1100

1200

1300

1400

1500

m/z, Da

665.5

Methylcobalamin Product of 673

673.0

971.5

358.9

1183.4

200

300

400

500

600

700

800

900

1000

1100

1200

1300

1400

1500

m/z, Da

147.1

358.9

04/05/2019