OMA Consulting Protocol Review: OMACON-2019-2

2.4.4.5 Once a day, the Biotest must be activated and calibrated before starting to perform the analysis. These two operations should also be carried out after the device has been inactive for long periods (30 minutes). 2.4.4.6 Once the Biosensor is set up, make sure that the measurement cuvette contains 10 mL of Measurement Solution and that all the components of the electrochemical cell are submerged. 2.4.5 Activation: Press activate, check that the cuvette is correctly fitted, and press CONTINUE to begin the activation process. 2.4.5.1 Wait until the device records the measurement target and it issues a sound signal. Inject the Measurement Standard into the measurement cuvette, using a micropipette, and wait until the process ends (see Table 5). 2.4.5.2 The message “Biotest activated. Ready for calibration” will appear on the screen 2.4.5.3 Change the content of the cuvette with 10 mL of new Measurement Solution.

Table 5: Addition volumes for activation, calibration and analysis 0-200 mg/L 0-2 g/L

0-6 g/L 650 µL 650 µL 650 µL 650 µL

LAC1 Calibration Standard LAC2 Calibration Standard Measurement Standard

2 mL 2 mL 2 mL 2 mL

2 mL 2 mL 2 mL 2 mL

Sample

2.4.6 Calibration: The calibration consists of 3 points and it is performed by means of adding the LAC1 Calibration Standard once and the LAC2

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