OMA Consulting Protocol Review: OMACON-2019-2

Calibration Standard twice to the measurement cuvette at three different times. 2.4.6.1 To trace the calibration line, the device will take into account the changes in intensity caused by the different additions and the relevant concentrations of each Calibration Standard. 2.4.6.2 Press Calibrate, fill the cuvette with 10 mL of new Measurement Solution and press CONTINUE. 2.4.6.3 Wait until the device issues a sound signal. Press CONTINUE and inject, in this order, the LAC1 Calibration Standard into the measurement cuvette (first addition (1/3)), using a micropipette (see Table 5). Note: It is important to add the Calibration Standard immediately after pressing the CONTINUE button to record the current intensity correctly at the time of adding it. 2.4.6.4 Wait until the device records the change in intensity and issues a sound signal. Press CONTINUE again and inject the LAC2 Calibration Standard into the measurement cuvette (second addition (2/3)) using a micropipette (see Table 5). 2.4.6.5 Wait until the device records the change in intensity and issues a sound signal. Press CONTINUE again and inject the LAC2 Calibration Standard again (third addition, (3/3)) using a micropipette (see Table 5). 2.4.6.6 As a result of the calibration, the parameters of the linear regression obtained in the interpolation of the three calibration points will appear on the screen.

2.4.6.7 R and m values equal to or over 0.9990 and 6.000, respectively, will be considered valid to proceed with the analysis. Typical values for b do not exceed 100.

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