OMA Consulting Protocol Review: OMACON-2019-2

• LAC Calibration Standards and Reagents:

The LAC Calibration Kit contains 6 tubes (CLAC1) of LAC1 powdered Calibration Reagent, 4 tubes (CLAC2) of LAC2 powdered Calibration Reagent, together with 2 empty tubes to prepare the LAC1 Calibration Standard, 2 empty tubes to prepare the LAC2 Calibration Standard and 3 empty tubes to prepare the Measurement Standard. The LAC1 and LAC2 Calibration Standards are prepared by diluting the LAC1 and LAC2 Calibration Reagents, respectively, and these are the reagents to be used to calibrate the equipment. The Measurement Standard is used to test the calibration status (see Table 3).

Table 3: Preparing the Calibration Reagents and Standards.

0-200 mg/l

0-2 g/l

0-6 g/l

LAC1 Calibration Reagent (CLAC1) LAC1 Calibration Standard LAC2 Calibration Reagent (CLAC2)

10 ml MS (*)

10 ml MS (*)

10 ml MS (*)

4 ml MS + 1ml CLAC1

4,9 ml MS + 0,1ml CLAC1

4,9 ml MS + 0,1ml CLAC1

5 ml MS (*)

5 ml MS (*)

5 ml MS (*)

9,75 ml MS + 0,25 ml CLAC2

9,75 ml MS + 0,25 ml CLAC2

9,25 ml MS + 0,75 ml CLAC2

LAC2 Calibration Standard

7,75 ml MS + 2 ml CLAC1 + 0,25 ml CLAC2

9,55 ml MS + 0,2 ml CLAC1 + 0,25 ml CLAC2

9,05 ml MS + 0,2 ml CLAC1 + 0,75 ml CLAC2

Measurement Standard

(*) Prepare it 24 hours before being used.

8