OMA Consulting Protocol Review: OMACON-2019-2

b) Activation and Calibration of the Biotest. Measurement Standard Analysis. Once a day, the Biotest has to be activated and calibrated before starting to perform the analysis. These two operations should also be carried out after the device has been inactive for long periods (over 2 hours). Once the Biosensor is set up (see Point 4.1.), make sure that the measurement cuvette contains 10 ml of Measurement Solution and that all the components of the electrochemical cell are submerged. 1.1. Press Activate, check that the cuvette is correctly fitted and press CONTINUE to begin the activation process. 1.2. Wait until the device records the measurement target and it issues a sound signal. Inject the Measurement Standard into the measurement cuvette, using a micropipette, and wait until the process ends (see Table 5). 1.3. Change the content of the cuvette with 10 ml of new Measurement Solution. The calibration consists on 3 points and it is performed by means of adding the LAC1 Calibration Standard once and the LAC2 Calibration Standard twice to the measurement cuvette at three different times. To trace the calibration line, the device will take into account the changes in intensity caused by the different additions and the relevant concentrations of each Calibration Standard. 2. Calibration: 1.Activation:

12