OMA Consulting Protocol Review: OMACON-2019-2

tube and fill it with Measurement Solution until the base of the Biotest is submerged. Once hydrated, the Biotest can be stored vertically at 3-8 o C for a maximum of 15 days.

2.4.3 Reagent Preparation:

2.4.3.1 LAC Measurement Solution (MS): Both LAC1 Measurement Kit and LAC2 Measurement Kit contain 8 tubes (MLAC1 or MLAC2). To prepare the Measurement Solution, which is needed to prepare

Reagents and Calibration Standards and to carry out the measurements, dilute the contents of one tube (MLAC1 or MLAC2) according to Table 2.

Table 2: Measurement Solution (MS) Preparation LAC1 Measurement Tube (MLAC1)

LAC2 Measurement Tube (MLAC2)

Volume of Distilled Water

250 mL

1 L

2.4.3.2 LAC Calibration Standards and Reagents: The LAC Calibration Kit contains 6 tubes of powdered CLAC1 Calibration Reagent, 4 tubes of powdered CLAC2 Calibration Reagent, along with 2 empty tubes to prepare the LAC1 Calibration Standard, 2 empty tubes to prepare the LAC2 Calibration Standard and 3 empty tubes to prepare the Measurement Standard. 2.4.3.2.1 CLAC1 and CLAC2 are prepared using measurement solution as outlined in Table 3. To prevent lose of reagent, introduce the pipette tip through the perforated stopper when adding the measurement solution. Store at 3-8 o C for up to 7 days. 2.4.3.2.2 The LAC Calibration Standards (LAC1, LAC2 and

Measurement Standard) are prepared daily by diluting the CLAC1 and CLAC2 Calibration Reagents. These standards are used to calibrate the equipment. For milk products, dilute the standards according to the specifications in Table 3.

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