OMA Consulting Protocol Review: OMACON-2019-2

Table 3: Preparing the Calibration Reagents and Standard 0-200 mg/L 0-2 g/L 0-6 g/L

LAC1 Calibration Reagent (CLAC1) LAC1 Calibration Standard LAC2 Calibration Reagent (CLAC2) LAC2 Calibration Standard

10 mL MS (*)

10 mL MS (*)

10 mL MS (*)

4 mL MS + 1 mL CLAC1

4.9 mL MS + 0.1 mL CLAC1

4.9 mL MS + 0.1 mL CLAC1

5 mL MS (*)

5 mL MS (*)

5 mL MS (*)

9.75 mL MS + 0.25 mL CLAC2

9.75 mL MS + 0.25 mL CLAC2

9.75 mL MS + 0.25 mL CLAC2

7.75 mL MS + 2mL CLAC1 + 0.25 mL CLAC2

9.55 mL MS + 0.2 mL CLAC1 + 0.25 mL CLAC2

9.05 mL MS + 0.2 mL CLAC1 + 0.75 mL CLAC2

Measurement Standard

2.4.3.2.3 The storage conditions of each of the prepared reagents are specified in Table 4.

Table 4: Reagent Storage Conditions Biotest CLAC1/ CLAC2

Measurement Reagent

Standards

Sample

Dry

3-8 o C/ e.d 3-8 o C/ 15 days

r.t./e.d/*

-

r.t./e.d/*

-

Hydrated

3-8 o C/ 7 days

Daily

r.t./15 days

2 hours

e.d.: until expiration date r.t.: room temperature *: keep in a dark and dry place

2.4.3.2.4 If other milk products are to be analyzed, with a glucose content different from ~23 g/l (hydrolysis of the total content of lactose of milk), the LAC1

Calibration Standard and the Measurement Standard must be changed in order to reflect the different glucose content. Use the following formula:

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