OMAMAN-47-ERPGlutenAssays-MeetingBook

ERP for Gluten Assays 64

PREPLATE 1 2 3 4 5 6 7 1 2 and 3 4 and 5 6 7 A STD 1 2 2 10 10 18 STD 1 A STD 1 2 10 18 STD 1

B STD 2 3 3 11 11 18 STD 2 B STD 2 3 11 18 STD 2

C STD 3 4 4 12 12 19 STD 3 C STD 3 4 12 19 STD 3

D STD 4 5 5 13 13 19 STD 4 D STD 4 5 13 19 STD 4

E STD 5 6 6 14 14 20 STD 5 E STD 5 6 14 20 STD 5

F STD 6 7 7 15 15 20 STD 6 F STD 6 7 15 20 STD 6 G 1 8 8 16 16 21 G 1 8 16 21

H 1 9 9 17 17 21 H 1 9 17 21

OD-Values obtained (absorbance units) (platereader data) Specify plate reader 1 2 3 4 5 6 7 IMPORTANT REMARK A If samples show OD values higher than STD 6 B dilute further at 1:5 (1+4) with the following buffer

Use the Excel sheet "Samples with OD > STD6" to document the analysis of further diluted samples

C - 1% Cocktail (patented), depending on the used extraction D - 3% of an 80% ethanol solution E - 96% buffer

F e.g. 50 µl Cocktail, 150 µl 80% ethanol and 4800 µl buffer. G Refer to the Instructions for collaborators H

Plate layout and results run 1

Run 1 (samples 1 to 21) Plate layout

Results (OD values)

DATA RETURN SHEET: RUN 1 (SAMPLES 1 to 21) RIDASCREEN ® Total Gluten R7041 Lab Code Date YYYY-MM-DD pipetting scheme for a plate with 7 strips

STD Standard (for number see label on the vial) 1 to 21 sample number

December 6, 2018