OMAMAN-47-ERPGlutenAssays-MeetingBook

ERP for Gluten Assays 65

PREPLATE 1 2 3 4 5 6 7 1 2 and 3 4 and 5 6 7 A STD 1 23 23 31 31 39 STD 1 A STD 1 23 31 39 STD 1

F STD 6 28 28 36 36 41 STD 6 F STD 6 28 36 41 STD 6 G 22 29 29 37 37 42 G 22 29 37 42

D STD 4 26 26 34 34 40 STD 4 D STD 4 26 34 40 STD 4

E STD 5 27 27 35 35 41 STD 5 E STD 5 27 35 41 STD 5

C STD 3 25 25 33 33 40 STD 3 C STD 3 25 33 40 STD 3

B STD 2 24 24 32 32 39 STD 2 B STD 2 24 32 39 STD 2

H 22 30 30 38 38 42 H 22 30 38 42

OD-Values obtained (absorbance units) (platereader data) Specify plate reader 1 2 3 4 5 6 7 IMPORTANT REMARK A If samples show OD values higher than STD 6 B dilute further at 1:5 (1+4) with the following buffer

Use the Excel sheet "Samples with OD > STD6" to document the analysis of further diluted samples

C - 1% Cocktail (patented), depending on the used extraction D - 3% of an 80% ethanol solution E - 96% buffer

F e.g. 50 µl Cocktail, 150 µl 80% ethanol and 4800 µl buffer. G Refer to the Instructions for collaborators H

Plate layout and results run 2

Plate layout

Results (OD values)

Run 2 (samples 22 to 42)

DATA RETURN SHEET: RUN 2 (SAMPLES 22 to 42) RIDASCREEN ® Total Gluten R7041 Lab Code Date YYYY-MM-DD pipetting scheme for a plate with 7 strips

STD Standard (for number see label on the vial) 22 to 42 sample number

December 6, 2018