OMB First to Final Action Recommendation Checklist 11-12-18

ERP - GLUTEN ASSAYS: AOAC 2015.05 FAR

Table 7. Summary of the DLA PT round 10/2016 characteristics; details for calculation are given in  Annex 5. 

Characteristics

sample A

sample C

mg/kg

mg/kg

Number of results Number of outliers

15

16

1

0

Median

250 251 54.4 62.7 125 376 0.85 17.2 0.28

18.6 20.4 7.95 5.11 10.2 30.6

Robust mean X pt

Robust standard deviation S* Target standard deviation  pt lower limit target range (X pt - 2  pt ) upper limit target range (X pt + 2  pt )

Quotient S*/  pt

1.6

Standard uncertainty U (Xpt)

2.48 0.49

Quotient U (Xpt) /  pt

Number of results in target range

14

14

Percent in target range

93%

88%

4. Conclusion  All data presented in chapter 3 provide evidence that the OMA 2015.05 First Action (RIDASCREEN®  Gliadin competitive R7021) is suitable for measurement of partially hydrolyzed gliadin and secalin in  syrup and sourdough, respectively. Furthermore, data from a former collaborative test that led to  AOAC OMA First Action of this system showed that assay characteristics precision and recovery – especially for beer‐ did not change over the past three years.   The method developers kindly ask the ERP Gluten to propose this method for Final Action to the Official  Method board.  

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