OMB First to Final Action Recommendation Checklist 11-12-18

ERP - GLUTEN ASSAYS: AOAC 2015.05 FAR

ANNEX 1 

PWG (gliadin) in syrup

target mg/kg blank blank blank blank blank blank target mg/kg

measured

mg/kg

2.17 1.65 2.43 1.69 1.27 1.69 58.5 51.2 48.6 40.6 49.3 44.5 32.5 27.1 29.8 26.1 31.2 31.8 22.8 23.7 24.7 25.0 22.4 24.6 14.0 15.1 15.4 15.5 15.0 15.9 12.4 12.6 12.5 12.6 14.8 15.5 10.3 11.4 11.1 11.9 11.0 9.96 5.16 4.69 4.25 3.81 4.17 4.55

mean

1.82 mg/kg 0.42 mg/kg

SD CV

22.8%

measured

blank corrected

recovery

mg/kg

mg/kg

%

30 30 30 30 30 30 21 21 21 21 21 21 16 16 16 16 16 16 12 12 12 12 12 12

56.7 49.4 46.8 38.8 47.4 42.6 30.7 25.3 27.9 24.3 29.4 30.0 21.0 21.9 22.9 23.2 20.6 22.8 12.1 13.3 13.6 13.6 13.1 14.0 10.6 10.8 10.6 10.8 12.9 13.7 8.51 9.61 9.25 10.1 9.14 8.14 3.34 2.87 2.43 1.99 2.35 2.73

189 165 156 129 158 142 146 120 133 116 140 143 131 137 143 145 129 142 101 111 113 114 109 117 118 120 118 120 144 152 142 160 154 169 152 136 111

mean

46.9 mg/kg 6.11 mg/kg

SD CV

13.0% 156%

mean rec.

mean

27.9 mg/kg 2.60 mg/kg

SD CV

9.3% 133%

mean rec.

mean

22.1 mg/kg 1.07 mg/kg

SD CV

4.9% 138%

mean rec.

mean

13.3 mg/kg 0.65 mg/kg

SD CV

4.9% 111%

mean rec.

9 9 9 9 9 9 6 6 6 6 6 6 3 3 3 3 3 3

mean

11.6 mg/kg 1.37 mg/kg

SD CV

11.8% 129%

mean rec.

mean

9.13 mg

SD CV

0.72 mg/kg

7.9% 152%

mean rec.

96 81 66 78 91

mean

2.62 mg/kg 0.47 mg/kg

SD CV

17.9%

mean rec. 87% All values are given in mg/kg prolamins. Concentrations below standard 2 (5 mg/kg) were extrapolated using  2 nd  order polynomial function. 

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