OMB First to Final Action Recommendation Checklist 11-12-18

ERP - GLUTEN ASSAYS: AOAC 2015.05 FAR

ANNEX 2 

Partially hydrolyzed gliadin in syrup

target mg/kg blank blank blank blank blank blank target mg/kg

measured

mg/kg

2.17 1.65 2.43 1.69 1.27 1.69 19.7 20.5 16.3 18.1 18.2 16.3 13.3 13.1 12.8 13.6 15.0 15.9 12.2 10.8 10.6 10.0 12.3 12.5 8.49 8.33 8.46 9.17 9.59 9.53 8.69 8.86 7.57 9.26 9.09 8.84 7.69 7.64 7.04 6.88 8.06 7.63 3.23 3.90 3.67 4.43 3.23 3.48

mean

1.82 mg/kg 0.42 mg/kg

SD CV

22.8%

measured

blank corrected

recovery

mg/kg

mg/kg

%

30 30 30 30 30 30 21 21 21 21 21 21 16 16 16 16 16 16 12 12 12 12 12 12

17.9 18.6 14.5 16.3 16.4 14.5 11.5 11.3 11.0 11.8 13.2 14.1 10.3 8.99 8.74 8.21 10.5 10.7 6.67 6.51 6.64 7.35 7.77 7.71 6.87 7.04 5.75 7.44 7.27 7.02 5.87 5.82 5.22 5.06 6.24 5.81 1.41 2.08 1.85 2.61 1.41 1.66

60 62 48 54 55 48 55 54 52 56 63 67 65 56 55 51 66 67 56 54 55 61 65 64 76 78 64 83 81 78 98 97 87 84 97 47 69 62 87 47 55

mean

16.3 mg/kg 1.72 mg/kg

SD CV

10.5%

mean rec.

54%

mean

12.1 mg/kg 1.25 mg/kg

SD CV

10.3%

mean rec.

58%

mean

9.58 mg/kg 1.06 mg/kg

SD CV

11.0%

mean rec.

60%

mean

7.11 mg/kg 0.57 mg/kg

SD CV

8.0% 59%

mean rec.

9 9 9 9 9 9 6 6 6 6 6 6 3 3 3 3 3 3

mean

6.90 mg/kg 0.60 mg/kg

SD CV

8.7% 77%

mean rec.

mean

5.67 mg/kg 0.44 mg/kg

SD CV

104

7.8% 95%

mean rec.

mean

1.84 mg/kg 0.46 mg/kg

SD CV

25.0%

mean rec. 61% All values are given in mg/kg prolamins. Concentrations below standard 2 (5 mg/kg) were extrapolated using  2 nd  order polynomial function. 

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