OMB First to Final Action Recommendation Checklist 11-12-18

ERP - GLUTEN ASSAYS: AOAC 2015.05 FAR

ANNEX 3 

Secalin in sourdough

target mg/kg blank blank blank blank blank blank target mg/kg

measured

mg/kg

0.18 0.31 0.89 0.60 0.92 0.61 40.4 40.5 47.6 41.6 48.2 52.2 19.7 29.8 22.4 20.9 23.4 19.9

mean

0.59 mg/kg 0.30 mg/kg

SD CV

50.9%

measured

blank corrected

recovery

mg/kg

mg/kg

%

15 15 15 15 15 15 10 10 10 10 10 10

39.8 39.9 47.0 41.0 47.6 51.6 19.1 29.2 21.8 20.3 22.8 19.3

265 266 313 273 317 344 191 292 218 203 228 193 132 155 138 137 142 137 120 128 130 131 129 145

mean

45.1 mg/kg 4.93 mg/kg

SD CV

10.9% 297%

mean rec.

mean

22.7 mg/kg 3.78 mg/kg

SD CV

16.7% 221%

mean rec.

5 5 5 5 5 5

7.2 8.4 7.5 7.4 7.7 7.4 3.6 3.8 3.8 3.9 3.8 4.2

6.6 7.8 6.9 6.9 7.1 6.9 3.0 3.2 3.3 3.3 3.2 3.6

mean

7.60 mg/kg 0.40 mg/kg

SD CV

5.3% 140%

mean rec.

2.5 2.5 2.5 2.5 2.5 2.5

mean

3.85 mg/kg 0.20 mg/kg

SD CV

5.3%

mean rec. 130% All values are given in mg/kg prolamins. Concentrations below standard 2 (5 mg/kg) were extrapolated using  2 nd  order polynomial function. 

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