OMB First to Final Action Recommendation Checklist 11-12-18

ERP - GLUTEN ASSAYS: AOAC 2015.05 FAR

ANNEX 4 

Partially hydrolyzed secalin in sourdough

target mg/kg blank blank blank blank blank blank target mg/kg

measured

mg/kg

0.18 0.31 0.89 0.60 0.92 0.61 33.8 33.5 32.4 37.1 35.9 36.9 23.5 22.8 23.7 21.2 24.5 23.5 11.3 12.0 11.1 11.4 10.1

mean

0.59 mg/kg 0.30 mg/kg

SD CV

50.9%

measured

blank corrected

recovery

mg/kg

mg/kg

%

15 15 15 15 15 15 10 10 10 10 10 10

33.2 32.9 31.8 36.5 35.3 36.3 22.9 22.2 23.1 20.7 23.9 22.9 10.7 11.4 10.5 10.8

222 219 212 244 236 242 229 222 231 207 239 229 215 228 210 216 190 183 117 175 185 193 221 191

mean

34.9 mg/kg 1.97 mg/kg

SD CV

5.6% 229%

mean rec.

mean

23.2 mg/kg 1.11 mg/kg

SD CV

4.8% 226%

mean rec.

5 5 5 5 5 5

mean

10.9 mg/kg 0.86 mg/kg

SD CV

9.5 9.2 2.9 4.4 4.6 4.8 5.5 4.8

7.8% 207%

9.7 3.5 5.0 5.2 5.4 6.1 5.4

mean rec.

2.5 2.5 2.5 2.5 2.5 2.5

mean

5.10 mg/kg 0.87 mg/kg

SD CV

17.0%

mean rec. 180% All values are given in mg/kg prolamins. Concentrations below standard 2 (5 mg/kg) were extrapolated using  2 nd  order polynomial function. 

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