OMB First to Final Action Recommendation Checklist 11-12-18

( 2 8VLQJ WKH UHJUHVVLRQ GDWD IURP WKH DSSURSULDWH PDWUL[ matched calibration curve, calculate the concentration of each pesticide found in the samples. ( 3 ) If a validated computer system is not being used for calculations, follow the steps below: ( a 0HDVXUH WKH SHDN DUHD RI HDFK UHVSHFWLYH VWDQGDUG OHYHO IRU HDFK SHVWLFLGH DQG WKH SHDN DUHD RI FRUUHVSRQGLQJ ,67' ( b ) Calculate the ratio of the analyte response to that of the ,67' ( c ) Run a linear regression analysis using the ratio of each SHVWLFLGH DW ¿YH GLIIHUHQW OHYHOV ZLWK QR ZHLJKWLQJ RU [ ZHLJKWLQJ ZKHUH [ FRQFHQWUDWLRQ ( d 0HDVXUH WKH SHDN DUHD RI HDFK SHVWLFLGH IRXQG LQ WKH VDPSOH DQG WKH SHDN DUHD RI FRUUHVSRQGLQJ ,67' ( e ) Calculate the amount of each pesticide in the solution injected from the standard curve. ( f ) Calculate the amount of each pesticide present in the sample. Test results should be reported to two decimal places or four VLJQL¿FDQW GLJLWV Reference: J. AOAC Int . 98 , 1428(2015) '2, MDRDFLQW Posted: October 13, 2015

( 2 0HDVXUH WKH LRQ DEXQGDQFHV IRU WKH TXDOL¿HU LRQV IRU WKH GHWHFWHG SHVWLFLGHV DQG YHULI\ WKDW WKH\ DUH ZLWKLQ WKH H[SHFWHG OLPLWV ,I WKH SHDNV PDWFK WKH SUHVHQFH RI WKH SHVWLFLGH LV FRQ¿UPHG See Table 2014.09H . (b) Quantitative calculations.— ( 1 ) Use instrument data SURFHVVLQJ VRIWZDUH IRU *& 06 6,0 *&06 06 DQG RU /& 06 06 WR FDOFXODWH D UHVSRQVH UDWLR PHDVXUHG DEXQGDQFH RI SHVWLFLGH PHDVXUHG DEXQGDQFH RI KHSWDFKORU HSR[LGH IRU *& DQG FKORUS\ULIRV PHWK\O IRU /& DQG FRQVWUXFW D ¿YH SRLQW PDWUL[ matched calibration curve of response ratio versus concentration of pesticide in standard solution. + 5HFRPPHQGHG PD[LPXP SHUPLWWHG WROHUDQFHV IRU UHODWLYH LRQ LQWHQVLWLHV XVLQJ D UDQJH RI PDVV VSHFWURPHWULF WHFKQLTXHV Relative intensity RI EDVH SHDN GC/MS (relative), % GC/MS/MS, LC/MS/MS (relative), % >50 ±10 ±20 >20–50 ±15 ±25 >10–20 ±20 ±30 ” ±50 ±50 7DEOH

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