OMB_Meeting_Book-11-2016

AOAC OMB Teleconference Materials

127

Yang Zhou, Ph.D., Curriculum Vitae

x Role as investigator and analytical expert for six human clinical projects, provides quality control and technical support for analytical data. x Prepare written reports, summarized experimental data and conclusions and submitted to Unilever x Manages and mentors lab members including graduate students, technician, and intern x Discuss with project members about subject compliance, data validation, methodology and statistical analysis Accomplishments: x Completed six human clinical projects and discovered the quantitative effects of various factors on nutrition absorption. x Developed and implemented methods for analysis of carotenoids and micronutrients in food samples, including fruits, vegetables, beverages, cereal grains and vegetable oils x Performed HPLC with UV/VIS, fluorescence, and high-sensitivity electrochemical (CoulArray®) detection of micronutrients and bioactive components x Produced monoclonal antibody for ȕ-carotene - 15,15'-monooxygenase (BCMO1), quantified BCMO1 expression in caco-2 cell western blot Research Intern, Pioneer Hi-Bred/DuPont, Inc. Johnston, IA 2007-2008 x Screened for novel genes with roles in yield enhancement and drought resistance in maize and soybean, comprehensively characterized the effect of genes on traits in corn and soybean x Cultured transgenic Arabidopsis and evaluated the effect of target genes on phenotype and traits, developed transgenic maize and soybean with tissue bombardment x Developed and implemented various bioassays for determining cytokinin in plant tissues x Performed RT-PCR to quantify the expression of target genes, extracted DNA and RNA from various plant tissues x DNA manipulation, protein expression and purification: – Constructed various protein mutants. – Expressed mutated proteins in E. Coli and mammalian cells and purified protein using gel chromatography x Enzyme kinetics of FBPase subunit exchange with fluorescence quantification: – Proven subunit exchange of FBPase caused by oriented and ordered dissocation of tetramer and dimer. – Established innovative methods for examining association/dissociation constants by quantifying the self-quenching of fluorescence signal. – Developed kinetic model of fructose-1,6-bisphosphatase (FBPase) subunit exchange and defined rate constants of enzyme association/dissociation x X-ray crystallographic determination of substrate binding in ADSS: – Crystallized adenylosuccinate synthetase (ADSS) and its mutants under various states to identify the structural factor on recognizing L-aspartate – Delineated the molecular basis whereby valine 273 organizes the L-aspartate binding site x Computational validation of ligand specificity in proteins: – Proved cavitation as a mechanism of substrate recognition in proteins – Predicted affinity change resulting from cavity presence by deriving the molecular potential energy under Lennard-Jones 12-6 definition – Validated protein functions and protein-substrate affinity through enzyme kinetics. Graduate study, Iowa State University, Ames, IA

2001-2007

2

11/09/2016