OMB Meeting Book_9-11-14

Question 1 Comment 1

In your experience using the method, does the method perform according to the method’s applicability User Comments No for phosphatidylinositol determination: the protocol of extraction and sample clean up is well described but very tricky. We didn’t manage to analyze the SRM1849a (no pik). In a lab for routine analysis it will be impossible to perform this test. If it is of interest another way should be use.

2

Paragraph D. Procedure / 3. HPLC analysis/ Instrument start up: In our opinion it’s necessary to use helium to pressurize mobile phase. In our opinion, “Area where temperature fluctuations will be minimal throughout the run” is not precise enough. The oven temperature we use for the HPLC column is 30°C and the resolution is much better.

3

Paragraph C. Preparation of reagent: Mobile phase Expiration 1 month seems to be very long (the temperature of storage is not indicated). Solutions can be stored at 2-30°C(very large?)

4 5

Yes It is change in part of the IC instrument in PAD setting base on MLT organizer

6

Change in step heat of bound-inositol from 110 to 130 C base on MLT organizer

7 8

Yes

No. The hydrolysis temperature written in the method had to be changed before the data for the phosphatidylinositol practice sample was accepted. In addition, the recovery for the free myo-inositol for certain matrices were affected by changes in the sample size.

Question 2

In your experience with the method, are there any safety concerns identified while using or regarding u

Comment 1 2

User Comments NO

The sample cleanup for phosphatidylinositol determinations needs to be performed in a fume hood. 2) After hydrolysis, 50% NaOH needs to be added very slowly to avoid splash of the solution, or use more diluted NaOH solution for the neutralization.