OMB Meeting Book_9-11-14

Vitamin A & E - MLT For ERP Use Only DO NOT DISTRIBUTE

SIMULTANEOUS DETERMINATION OF 13-CIS AND ALL-TRANS VITAMIN A PALMITATE (RETINYL PALMITATE), VITAMIN A ACETATE (RETINYL ACETATE), AND TOTAL VITAMIN E (Α-TOCOPHEROL AND DL-Α-TOCOPHEROL ACETATE) IN INFANT FORMULA AND ADULT NUTRITIONALS BY NORMAL PHASE HPLC

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AOAC 2012.10

Jan 2014

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AOAC MLT Study

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A. INTRODUCTION

Vitamin A (retinol) is an essential nutrient for normal vision, and teeth and bone formation. An inadequate intake of Vitamin A causes Xerophthalmia, resulting in blindness, stunted growth and possible death. An overdose of vitamin A is damaging to infants and adults. Vitamin A can exit in several isomeric forms and as esters. Retinyl palmitate will isomerize under thermal and photochemical stress to a variety of cis-isomers, of which 13-cis is the most common and most active (75% of trans). Other isomers have reduced vitamin A activity. In this method no distinction is made between the bioactives of the isomers, instead all are summed against the trans isomer to give the total vitamin A concentration. Vitamin E is an antioxidant, protecting many other biochemical form damage by active oxygen and other free radicals. Vitamin E has eight active forms of which α-tocopherol is usually considered because it is the most active and abundant form.

B. SCOPE

The method is applicable to the simultaneous quantitative determination of total Vitamin E (dl-α- tocopherol and dl-α-tocopherol acetate), vitamin A palmitate and vitamin A acetate (cis-and trans- isomers) present in milk- and soy-based infant formula and adult nutritionals and formulas containing hydrolyzed protein.

The analytical range of the method is as follows:

Vitamin A, Retinyl Palmitate; 2 – 450 mcg/100 g reconstituted.

Vitamin A Acetate; 2 – 450 mcg/100 g reconstituted.

DL-α-Tocopherol Acetate; 0.02 — 9.4 mg/100g reconstituted.

DL-α-Tocopherol; 0.03 – 8.0 mg/100 g reconstituted.

C. REFERENCE

1. AOAC Official Method 2012.10: Simultaneous Determination of 13-cis and all-trans Vitamin A Palmitate (Retinyl Palmitate), Vitamin A Acetate (Retinyl Acetate), and Total Vitamin E (dl- α-Tocopherol and dl-α-Tocopherol Acetate) in Infant formula and Adult Nutritionals.

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