OMB Meeting Book_9-11-14

Vitamin A & E - MLT For ERP Use Only DO NOT DISTRIBUTE

SIMULTANEOUS DETERMINATION OF 13-CIS AND ALL-TRANS VITAMIN A PALMITATE (RETINYL PALMITATE), VITAMIN A ACETATE (RETINYL ACETATE), AND TOTAL VITAMIN E (Α-TOCOPHEROL AND DL-Α-TOCOPHEROL ACETATE) IN INFANT FORMULA AND ADULT NUTRITIONALS BY NORMAL PHASE HPLC

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AOAC 2012.10

Jan 2014

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AOAC MLT Study

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Retinyl Palmitate and Retinyl Acetate

For the purpose of this MLT the trans and 13-cis isomers need to be reported separately. To do this the 13- cis -isomer is calculated with the same slope and intercept as is used to quantify the trans isomer. This can be done by the HPLC software or using an excel spreadsheet.

Report the 13-Cis and trans retinyl palmitate and 13-cis and trans retinyl acetate separately, as mcg/100g for powder and mcg/100mL for liquid presentations.

dl-α-tocopherol and dl-α-tocopherol acetate

dl-α-tocopherol and dl-α-tocopherol acetate are reported separately. Integration by height is recommended.

Report dl-α-tocopherol and dl-α-tocopherol acetate separately as mg/100g for powder and mg/100mL for liquid presentations.

Figure 1: Vitamin A palmitate and vitamin A acetate calibration standard.

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