OMB Meeting Book_9-11-14

Note : LC-PUFA is the generic name to describe the long-chain polyunsaturated fatty acids, but these fatty acids are also included into PUFA(i.e., arachidonic, ecosapentaenoic, and docosahexaenoic acids). ( 6 ) Omega-3 fatty acid (ω-3) .—Omega-3 fatty acid is the sum of cis PUFA having the first double bonds at carbon (n-3) from the terminal methyl group. ( 7 ) Omega-6 fatty acid (ω-6) .—Omega-6 fatty acid is the sum of cis PUFA having the first double bonds at carbon (n-6) from the terminal methyl group. ( 8 ) Omega-9 fatty acid (ω-9) .—Omega-3 fatty acid is the sum of cis PUFA having the first double bonds at carbon (n-9) from the terminal methyl group. ( b ) Individual fatty acids . ( 1 ) Linoleic acid (LA) .—LA is an essential fatty acid; an 18 carbon fatty acid containing two double bonds at carbons 9 and 12. ( 2 ) Linolenic acid (ALA) also called α-linolenic acid .—ALA is an essential fatty acid; an 18 carbon fatty acid containing three double bonds at carbons 9, 12, and 15. ( 3 ) Arachidonic acid (ARA) .—ARA is not an essential fatty acid; a 20 carbon fatty acid containing four double bonds at carbons 5, 8, 11, and 14. ( 4 ) Ecosapentaenoic acid (EPA) .—EPA is a semi-essential fatty acid (essential for pregnant woman and infant); a 20 carbon fatty acid containing five double bonds at carbons 5, 8, 11, 14, and 17. ( 5 ) Docosahexaenoic acid (DHA) .—DHA is a semi-essential fatty acid (essential for pregnant woman and infant); a 22 carbon fatty acid containing six double bonds at carbons 4, 7, 10, 13, 16, and 19. Reference: J. AOAC Int . (future issue)

could be the following: Incomplete transesterification, partial degradation of one internal standard, or matrix effect. J. Annex A: Groups or Class of Fatty Acids and Individual Fatty Acids ( a ) Group or class of fatty acid . ( 1 ) Trans fatty acids (TFA) .—TFA is the sum of fatty acids containing one or more nonconjugated double bonds in trans configuration (only C18:1, C18:2, and C18:3 trans are included in the sum). Note : Presence of other trans isomers naturally present in milk fat have been reported in literature (e.g., C16:1), but their contributions have no significant influence on the total amount of trans in milk products. In addition, their identification are complex especially because these isomers are frequently interfered with other isomers (e.g., cis , iso , and anteiso ) and need preliminary separation technique. ( 2 ) Conjugated linoleic acids (CLA) .—CLA are the sum of octadecadienoic acids containing conjugated double bonds in cis or trans configuration; mainly cis -9, trans -11 octadecadienoic acid (i.e., rumenic acid). ( 3 ) Saturated fatty acids (SFA) .—SFA are the sum of all fatty acids which contain no double bonds. ( 4 ) Monounsaturated fatty acids (MUFA) .—MUFA are the sum of all fatty acids containing one double bonds in cis configuration. ( 5 ) Polyunsaturated fatty acids (PUFA) .—PUFA are the sum of all fatty acids containing two or more double bonds in cis configuration.

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