OMB Meeting Book_9-11-14

ISO/WD

5.12 C11:0 FAME/C13:0 TAG standard solution

Into a 250 ml volumetric flask, weigh to the nearest 0,1 mg about 500 mg of tritridecanoin and 500 mg of methyl undecanoate. Dissolve and make up to the mark with MTBE.

Stored in the dark at 4 °C this solution is stable for one week. Allow the solution to come to room temperature before use.

This solution volume is sufficient to analyze approx. 40 samples or more. In case or smaller number of analysis, standard weights and volume of solvent can be adapted accordingly.

5.13 Octadecenoic acid methyl ester , cis/ trans -isomers mixture of C18:1 with trans -4 to trans -16 (all isomers) and principal cis isomers. Concentration 2,5 mg/ml in Methylene Chloride.

NOTE

This standard is commercially available from Sigma-Aldrich (Cat. 40495-U).

5.14 Linoleic acid methyl ester , cis / trans -isomers mixture of C18:2 n-6 with trans -9, trans -12-octadecadienoic acid (50%), cis -9, trans -12-octadecadienoic acid (20%), trans -9, cis -12-octadecadienoic acid (20%) and cis -9, cis - 12-octadecadienoic acid (10%). Concentration 10 mg/mL in Methylene Chloride.

NOTE Standard commercially only available from Supelco Inc (Cat. 47791) 1) .

5.15 Linolenic acid methyl ester , cis / trans isomer mixture of C18:3 n-6 with cis -9, cis -12, cis -15- Octadecatrienoic acid methyl ester ~ 3 % (w/w), cis -9, cis -12, trans -15-Octadecatrienoic acid methyl ester ~ 7 % (w/w), cis -9, trans -12, cis -15-Octadecatrienoic acid methyl ester ~ 7 % (w/w), cis -9, trans -12, trans -15- Octadecatrienoic acid methyl ester ~ 15 % (w/w), trans -9, cis -12, cis -15-Octadecatrienoic acid methyl ester ~ 7 % (w/w), trans -9, cis -12, trans -15-Octadecatrienoic acid methyl ester ~ 15 % (w/w), trans -9, trans -12, cis -15- Octadecatrienoic acid methyl ester ~ 15 % (w/w) and trans -9, trans -12, trans -15-Octadecatrienoic acid methyl ester ~ 30 % (w/w). Concentration 10 mg/mL in Methylene Chloride.

NOTE This standard is commercially available from Supelco Inc, (Cat. 47792). This standard contains all trans isomers but their abundance and ratio are different to those observed oils and fats.

5.16 Methyl octadecadienoate conjugated acids (mixture of C18:2 cis -9, trans -11 and cis -10, trans -12), of purity ≥99%.

5.17 Qualitative Cis / Trans FAME isomers standard mixture solution

For the RT identification of cis / trans isomers prepare a qualitative standard solution with the standard listed 5.13 to 5.16. All standards commercially available could be used. Into a 50 ml volumetric flask, add each standard isomer in equal proportion. Dissolve and make up to the mark with hexane. Dilute accordingly to the type of injector used.

5.18 Standard FAME mixture solution

a) Purchase individual FAME standard list as follow (of purity ≥99%);

Butyric acid methyl ester (C4:0), Caproic acid methyl ester (C6:0), Caprylic acid methyl ester (C8:0), Capric acid methyl ester (C10:0), Undecanoic acid methyl ester (C11:0), Lauric acid methyl ester (C12:0), Tridecanoic acid methyl ester (C13:0), Myristic acid methyl ester (C14:0), Myristoleic acid methyl ester (C14:1 n-5 or Δ9 cis),

1) Supelco Inc is an example of suitable product available commercially. This information is given for the convenience of the user of this International Standard and it does not constitute an endorsement by either ISO or IDF of this product.

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