OMB Meeting Book_9-11-14
ISO/WD
Table B.10 – Calculation of fatty acids (Example using Excel)
1
2
Response Repetition number
Results expressed in
x
x
factor
File name
gFA/100g
xx-xx-xx xx-xx-xx
related to Injection date
gFA/100g
301.80 285.00 total FA
Test portion (mg) product or oil
C-11:0
product (or oil)
9.620 9.6200
internal
C-11:0 FAME Internal standard amount (mg)
(FA%)
101400 101509 Mean 1
2 Mean SD RSD
standard C-11:0 FAME Internal standard area
9.375
(n=2)
(n=2)
9.375
%
C-13:0 (TAG) Internal standard amount (mg)
104944 105005
C-13:0 (TAG) Internal standard area
0 0.000
0.000 0.000
0.000 #DIV/0!
0
0.000
1.668 C-4:0
butyric
990 0.154
0.038 0.038
0.000 0.0
1047
0.038
1.282 C-6:0
caproic
0.377 0.377
0.000 0.0
11737 11100 1.537
0.377
1.121 C-8:0
caprylic
9780 1.248
0.305 0.306
0.001 0.2
10317
0.306
1.018 C-10:0
capric
2.157 2.164
0.006 0.3
76477 72560 8.816
2.160
0.959 C-12:0
lauric
0.961 0.965
0.002 0.2
35347 33520 3.930
0.963
0.919 C-14:0
myristic
180 0.022
0.005 0.005
0.000 1.0
193
0.005
0.939 C-14:1
myristoleic
N-5 (or w 5)
D9
620 0.073
0.018 0.018
0.000 0.0
656
0.018
0.915 C-15:0
pentadecanoic
0 0.000
0.000 0.000
0.000 #DIV/0!
0
0.000
0.927 C-15:1
pentadecenoic
N-5 (or w 5)
D10
5.953 5.982
0.021 0.3
223376 212207 24.352
5.968
0.894 C-16:0
palmitic
1840 0.216
0.053 0.053
0.000 0.0
1945
0.053
0.912 C-16:1
palmitoleic
N-7 (or w 7)
D9
840 0.096
0.024 0.024
0.000 0.1
890
0.024
0.887 C-17:0
margaric
259 0.030
0.007 0.007
0.000 0.3
275
0.007
0.896 C-17:1
heptadecenoic
0.934 0.939
0.003 0.3
35849 34052 3.822
0.937
0.870 C-18:0
stearic
1067 0.122
0.030 0.030
0.000 0.0
1128
0.030
0.881 C-18:1 TFA
total trans C18:1
8.706 8.749
0.030 0.3
334633 317905 35.613
8.727
0.868 C-18:1
oleic & others cis
N-9 (or w 9)
D9
2513 0.284
0.069 0.070
0.000 0.4
2642
0.070
0.878 C-18:2 TFA
total trans C18:2
3.802 3.822
0.014 0.4
146659 139350 15.555
3.812
0.866 C-18:2
linoleic
N-6 (or w 6)
D9,12
310 0.035
0.008 0.009
0.000 0.6
325
0.008
0.866 C-18:2 CLA N-7 (or w 7)
conjugated linoleic
D 9c/11t
232 0.026
0.006 0.006
0.000 1.0
242
0.006
0.881 C-18:3
gamma-linolenic
N-6 (or w 6)
D6,9,12
2040 0.228
0.056 0.056
0.000 0.4
2146
0.056
0.869 C-18:3 TFA
total trans C18:3
0.414 0.416
0.001 0.3
15922 15120 1.693
0.415
0.869 C-18:3
alpha-linolenic
N-3 (or w 3)
D9,12,15
3210 0.357
0.087 0.088
0.000 0.1
3390
0.087
0.857 C-20:0
arachidic
3620 0.406
0.100 0.099
0.000 0.1
3833
0.099
0.863 C-20:1
eicosenoic
N-9 (or w 9)
D11
245 0.027
0.007 0.007
0.000 1.7
253
0.007
0.861 C-20:2
eicosadienoic
N-6 (or w 6)
D11,14
222 0.025
0.006 0.006
0.000 1.5
230
0.006
0.868 C-20:3
eicosatrienoic (DHGLA)
N-6 (or w 6)
D8,11,14
0 0.000
0.000 0.000
0.000 #DIV/0!
0
0.000
0.868 C-20:3
eicosatrienoic
N-3 (or w 3)
D11,14,17
2385 0.267
0.065 0.066
0.000 0.6
2502
0.065
0.866 C-20:4
arachidonic (AA or ARA)
N-6 (or w 6)
D5,8,11,14
686 0.080
0.020 0.019
0.000 2.1
748
0.020
0.887 C-20:5
eicosapentanoic (EPA)
N-3 (or w 3)
D5,8,11,14,17
1709 0.189
0.046 0.047
0.000 0.7
1791
0.046
0.854 C-22:0
behenic
1234 0.139
0.034 0.034
0.000 0.7
1293
0.034
0.870 C-22:1
erucic
N-9 (or w 9)
D13
0 0.000
0.000 0.000
0.000 #DIV/0!
0
0.000
0.854 C-22:2
docosadienoic
N-6 (or w 6)
D13,16
1975 0.226
0.056 0.055
0.000 0.8
2112
0.055
0.875 C-22:6
docosahexaenoic (DHA)
N-3 (or w 3)
D4,7,10,13,16,19
1045 0.114
0.028 0.028
0.000 0.7
1095
0.028
0.839 C-24:0
lignoceric
395 0.044
0.011 0.011
0.000 1.7
408
0.011
0.869 C-24:1
nervonic
N-9 (or w 9)
D15
2415 0.274
0.067 0.067
0.000 0.5
2537
0.067
0.882 OFA
other fatty acids
921998 875626
100.000 24.450 24.562 24.506
0.079 0.3
Total
0.155 0.156
0.001 0.3
Total trans fatty acids
0.634
0.155
99.3 99.3
0.0 0.0
Transesterification performance (minimum 98.0 %)
99.3
26
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